ISSN:
1573-4986
Keywords:
1 H-NMR spectroscopy
;
intersected hybrid-type glycans
;
intersected multi-antennary glycans
;
dansyl derivative
;
ovalbumin
;
ovomucoid
;
ovotransferrin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Dansyl glyco-asparagines were prepared from a partially fractionated mixture of hen egg white glycoproteins. Reverse-phase high performance liquid chromatography (HPLC) on a silica-based octadecyl column yielded ten such derivatives in a virtually pure state. The detailed structures of the glyco-asparagines were identified by 500-MHz1H nuclear magnetic resonance (NMR) spectroscopy. Two of them were found to be of the oligomannosideN-type, four were of the intersected-hybridN-type and another four were of the intersected multi-antennaryN-type. In monogalactosylated, intersected structures the galactose residue was proved by1H-NMR to be attached in β(1–4)-linkage to the GlcNAcβ1-4Manα1–3 branch. Dansyl glyco-asparagines turned out to be suitable derivatives for1H-NMR spectroscopic analysis. The combination of HPLC and high-resolution NMR spectroscopy of such derivatives proved to be a powerful technique in studying the (micro-)heterogeneity of sugar chains in glycoproteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01049271
Permalink