ISSN:
1432-072X
Keywords:
Ozone
;
Yeast
;
Saccharomyces cerevisiae
;
ATP
;
Nucleotides
;
Permeability
;
Cytosolic enzymes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Treatment of a yeast suspension with ozone inactivates a number of cytosolic enzymes. Among 15 studied, the most drastic inactivation was found for glyceraldehyde-3-phosphate dehydrogenase and to lesser extents: NAD-glutamate dehydrogenase, pyruvate decarboxylase, phosphofructokinase-1 and NAD-alcohol dehydrogenase. Ozone treatment also effects the quantity of ATP and of other nucleoside triphosphates, reducing to about 50% of the initial value. The ATP missing in the cells appears in the medium. NAD and protein also accumulate in the medium suggesting that the yeast cells have been permeabilized. Permeabilization of the yeast cells by treatment with ozone preceeds the inactivation of glyceraldehyde-3-phosphate dehydrogenase and other cytosolic enzymes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00415269
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