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  • 1
    Electronic Resource
    Electronic Resource
    Re-expression of disease-characteristic features of non-bullous congenital ichthyosiform erythroderma (CIE) after graftig of the pathological keratinocyte cultures to athymic mice (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 16 (1989), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Epidermal keratinocytes separated from skin lesions of non-bullous congenital ichthyosiform erythroderma were investigated in an attempt at experimental reproduction of this keratinization disorder. In vitro studies on growth and differentiation of pathological keratinocytes isolated from the influence of the host's dermal and humoral components were performed using the immersed epidermal cell culture technique. Ten to 25- day-old confluent and stratified cultures were examined by means of photonic and electron microscopy, and stained with various differentiation markers for indirect immunofluorescence studies. The cultured epidermis showed low-grade differentiation and no clear-cut evenly distributed signs of the original disease. Grafting on congenitally athymic nude mice allowed further differentiation of the epidermal sheets and re-expression of the histologic and ultrastructural features of non-bullous congenital ichthyosiform erythroderma. Thus, the purely epidermal origin of this particular form of autosomal recessive ichthyosis could be confirmed. Large amounts of pathological keratinocytes generated from small skin biopsies may be used for experimental purposes after grafting on several athymic animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1989.tb00001.x
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural quantitation of desmosome and differentiation-related keratinocyte membrane antigen (1986)
    Springer
    Archives of dermatological research 278 (1986), S. 283-292 
    Details
    ISSN: 1432-069X
    Keywords: Keratinocyte differentiation ; Desmosome ; Immunogold labelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The keratinocyte membrane antigen KM 48 was defined by a new monoclonal antibody obtained after mouse immunization with normal human epidermal cell suspension. Specific reactivity of the antibody with desmosomal regions of keratinocyte cell membrane was demonstrated by immunoelectronmicroscopy. Langerhans cells, melanocytes, and indeterminate cells did not express the KM 48 antigen. Immunogold labelling permitted ultrastructural quantitation of KM 48 antibody binding on keratinocytes from various epidermal layers. A gradual increase in desmosome-related KM 48 antigen expression accompanied differentiation of keratinocytes durign their transit from basal to granular layer. Distribution of the antigen on individual cells was uneven. The upper pole of a keratinocyte facing overlying more differentiated cells was always found to be laden with an immunogold marker about twice that of the opposite, lower surface of the cell. The results support the previous reports on gradual development of desmosomes during epidermal cell maturation and open up new possibilities for keratinocyte differentiation studies. They also underline the virtues of the immunogold-labelling method used for cell-surface antigen tracing and quantitation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407739
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  • 3
    Electronic Resource
    Electronic Resource
    Quantitative analysis of T6-positive Langerhans cells in human skin cancers (1986)
    Springer
    Virchows Archiv 410 (1986), S. 57-63 
    Details
    ISSN: 1432-2307
    Keywords: Langerhans cells ; Basal cell carcinomas ; Squamous cell carcinomas ; Quantitative analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Epidermal Langerhans cells (LC) are required for antigen-presentation and for stimulating antigen-specific T cell activation. Similar functions may be important in the immune response to malignant skin tumours. Monoclonal anti-T6 antibody was used to examine LC population in basal and squamous cell carcinomas. Positive control labeling was performed with monoclonal anti-HLA-DR antibody. The number of T6-positive LC per mm2 of section was significantly decreased (p〈0.01) in the tumour group in comparison with a sex and age-matched control group. The number of sun-exposed and covered regions was taken into consideration in each respective group. Within the tumours, LC were found more frequently in the tumour periphery and in most differentiated tumour areas (horn pearls) than in the rest of the tumour mass. T6-positive LC were rarely found in the dermis. Moreover, LC exhibited morphological changes in specimens from tumours. Staining with anti-HLA-DR antibody revealed less numerous positive cells within tumour nests than labeling with OKT6. A relationship between T6-positive LC quantities and extent of HLA-DR-positive infiltrates around tumours could not be established. These results suggest that immunological surveillance of neoantigen-bearing tumour cells may be impaired in skin cancer. A reason for the reduced LC number may be an altered microenvironment in tumour tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00710906
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  • 4
    Electronic Resource
    Electronic Resource
    Keratin filament composition of human epidermal spinous and granular cell fractions selected by flow cytometric sorting (1987)
    Springer
    Archives of dermatological research 279 (1987), S. 273-275 
    Details
    ISSN: 1432-069X
    Keywords: Epidermal spinous cells ; Granular cells ; Keratins ; Flow cytometric sorting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417328
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