In:
FEBS Letters, Wiley, Vol. 220, No. 2 ( 1987-08-17), p. 383-386
Abstract:
A novel method is described for the extraction of DNAs from fungi and yeasts. Anhydrous hydrogen fluoride (HF) selectively cleaves their cell walls under mild conditions (for 5 min at 0°C), enabling the effective extraction of DNAs from organisms with a cell wall. A possible mechanism for this method concerning the selective cleavage of O‐glycosidic linkages in cell walls has been described previously [(1977) Anal. Biochem. 82, 289–309]. The extracted DNA is intact: in fact, the yeast DNA is directly applicable for restriction analysis and transformation of Escherichia coli .
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(87)80851-7
Language:
English
Publisher:
Wiley
Publication Date:
1987
detail.hit.zdb_id:
1460391-3
SSG:
12
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