In:
Scandinavian Journal of Immunology, Wiley, Vol. 30, No. 5 ( 1989-11), p. 597-603
Abstract:
The purpose of this study was to investigate interleukin 2 (IL‐2) and gamma interferon (IFN‐γ) production by purified CD4 + and CD8 + cells isolated from peripheral blood (PB) and synovial fluid (SF) of patients with rheumatoid arthritis (RA) and other inflammatory arthritides (non‐RA). CD4 + and CD8 + cells were selected positively by immunomagnetic separation. Supernatants of unstimulated CD4 + and CD8 + cells from both compartments did not contain any delectable IL‐2 or IFN‐γ, while supernatants of CD4 + and CDS I cells stimulated with phytohaemagglutinin and irradiated Raji cells mostly contained both cytokines. In vitro stimulated SF CD4 + cells gave supernatants with significantly less IL‐2 than supernatants from PBCD4 + cells, while in vitro stimulated SF CD4 + ‐cell supernatants contained significantly more IFN‐γ. SF CD4 + ‐cell supernatants contained significantly more IL‐2 than the parallel CD8 + supernatants, while there was no significant difference with regard to IFN‐γ content. The pattern of differences between SF ‐and PB‐derived T cells was the same for the two groups of patients, but the SF CD4 + cells from RA patients produced significantly less IL‐2 than the corresponding cells from the non‐RA group. The difference between SF and PB T cells with regard to lymphokine production is probably related to various degrees of in vivo pre‐activation. The results do not indicate a major T‐cell deficiency in relation to lymphokine production in RA.
Type of Medium:
Online Resource
ISSN:
0300-9475
,
1365-3083
DOI:
10.1111/sji.1989.30.issue-5
DOI:
10.1111/j.1365-3083.1989.tb02467.x
Language:
English
Publisher:
Wiley
Publication Date:
1989
detail.hit.zdb_id:
2020954-X
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