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  • 1
    Electronic Resource
    Electronic Resource
    Respirational activity of Chlorella fusca monitored by in vivo P-31 NMR (1985)
    Springer
    European biophysics journal 13 (1985), S. 89-97 
    Details
    ISSN: 1432-1017
    Keywords: 31P NMR ; respiration ; polyphosphate ; Chlorella ; intracellular pH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Energy metabolism during dark respiration of the green alga Chlorella fusca was investigated by 31P NMR spectroscopy. The kinetics of the transition from anaerobic to aerobic conditions (and vice versa) was followed with a temporal resolution of 16 s. This transition is accompanied by a shift of the cytoplasmic pH from 6.8 to 7.4, while the vacuolar pH remains constant. Simultaneously, an increase in the concentration of nucleoside-triphosphates and a decrease in the concentration of cytoplasmic orthophosphate take place, as well as the formation of “mobile” polyphosphates. The concentration of ATP and P i reach steady-state levels within 30 s. Upon the reverse transition, from aerobic to anaerobic conditions, steady-state concentrations are obtained only after 3 min.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00256529
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    In vivo P-31 NMR measurements of phosphate metabolism in Platymonas subcordiformis as related to external pH (1987)
    Springer
    European biophysics journal 14 (1987), S. 461-470 
    Details
    ISSN: 1432-1017
    Keywords: 31 P-NMR ; respiration ; photosynthesis ; intracellular pH ; polyphosphate ; pH regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The phosphate metabolism of Platymonas subcordiformis was investigated by 31P-NMR spectroscopy with special attention on the effect of external pH. Glycolyzing cells and cells energized by respiration or photosynthesis gave spectra dependent upon their metabolic state. The transition from deenergized to energized states is accompanied by a shift of cytoplasmic pH from 7.1–7.4, an increase of ATP level and-in well energized cells-the appearance of a new signal tentatively assigned to phosphoarginine. The spectra remain stable over a wide range of external pH. Cytoplasmic pH is well regulated in respiring cells for external pH in the range 5.3–12.3. The typical 0.4 units difference of internal pH in energized as compared to deenergized cells is not affected by external pH in the range 6–12. The intensity of a signal attributed to PEP is markedly increased at high external pH. pH regulation is less efficient below external pH of 6 in deenergized cells. Below pH 3.8 oxidative phosphorylation ceases. Upon raising cytoplasmic pH to 7.4 in deenergized cells polyphosphate chains start to disintegrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293255
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    Paper (German National Licenses)
    Fulltext
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