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  • Obligate methylotroph  (2)
  • 1985-1989  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Development of gene transfer systems in Methylobacillus flagellatum KT: Isolation of auxotrophic mutants (1987)
    Springer
    Archives of microbiology 149 (1987), S. 112-119 
    Details
    ISSN: 1432-072X
    Keywords: Methylobacillus flagellatum KT ; Obligate methylotroph ; Nitrosoguanidine mutagenesis ; Auxotrophic mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A collection of polyauxotrophic mutants of the obligate methylotroph Methylobacillus flagellatum KT was obtained. On the first step two stable auxotrophic mutants with a high requirement for amino acids supplements were isolated by treatment with nitrosoguanidine and selection on complete medium. Spontaneous variants of these mutants with a low requirement for nutrient supplements were the base for obtaining polyauxotrophic strains. It was shown, that the growth of mutants of M. flagellatum KT is inhibited by complete medium. Some amino acids and nucleotides are the inhibitor components of complete media. An approach for selection of auxotrophic mutants of individual genes was worked out on minimal medium. The optimal conditions for nitrosoguanidine mutagenesis of M. flagellatum KT were developed. The possible mechanisms of action of some of the nutrient supplements on the growth of M. flagellatum KT are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425075
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Mutants of the obligate methylothroph Methylobacillus flagellatum KT defective in genes of the ribulose monophosphate cycle of formaldehyde fixation (1988)
    Springer
    Archives of microbiology 149 (1988), S. 441-446 
    Details
    ISSN: 1432-072X
    Keywords: Methylobacillus flagellatum ; Obligate methylotroph ; Ribulose monophosphate cycle ; Temperature sensitive mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An approach to the isolation of mutants deficient in key enzymes of C1 metabolism was worked out for the obligate methylotroph Methylobacillus flagellatum KT. The isolation of mutants in genes of the ribulose monophosphate (RMP) cycle of formaldehyde assimilation and oxidation is based on selection of temperature sensitive (ts) mutants followed by enzymatic assays. Standard methods of determination of activities of hexulose phosphate synthase/hexulose phosphate isomerase (HPS/HPI), phosphoglucoisomerase (PGI), glucose 6-phosphate dehydrogenase (GPD) and 6-phosphogluconate dehydrogenase (GND) were modified for screening the ts-mutants for the presence of RMP keyenzyme activities. Among the 500 ts-mutants investigated, nine mutants were defective in PGI activity and two in GDP activity. The defective enzymes were characterized by a high rate of inactivation at increased temperatures. At 50° C the proteins studied were completely inactivated during 2 h, whereas the native enzymes maintained more than 80% activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425585
    Location Call Number Limitation Availability
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    Paper (German National Licenses)
    Fulltext
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