ISSN:
1432-2242
Keywords:
Chromosomes
;
Flow cytometry
;
Theoretical flow karyotypes
;
Spreadsheet software
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Theoretical flow karyotypes from both plant and mammalian species have been simply modelled using computer spreadsheet software. The models are based upon published values of relative DNA content or relative lengths of each of the chromosomes. From such data, the histograms of chromosome distribution have been simulated for both linear and logarithmic modules of a flow cytometer, and as a function of the coefficient of variation. Simulated and experimental histograms are compard for Nicotiana plumbaginifolia. This readily accessible exercise facilitates the planning and execution of flow cytometric analysis and sorting of chromosomes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00266200
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