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  • exocytosis  (2)
  • Digitonin permeabilization  (1)
  • 1985-1989  (3)
Document type
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Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Isolated neurosecretory nerve endings as a tool for studying the mechanism of stimulus-secretion coupling (1987)
    Springer
    Bioscience reports 7 (1987), S. 411-426 
    Details
    ISSN: 1573-4935
    Keywords: neurosecretion ; nerve endings ; stimulus-secretion coupling ; secretion ; exocytosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In the present paper we discuss the properties of a recently developed preparation of isolated neurosecretory nerve endings obtained from the rate neurohypophysis. These nerve terminals release two neurohormones, oxytocin and vasopressin, which are easily assayed by radioimmunoassay. Depolarization-induced secretion is dependent on the same parameters as those regulating release from the whole neural lobe. The isolated nerve endings can be permeabilized by means of digitonin; a treatment which gives direct access to the cytoplasm allowing the study of the minimal requirements for inducing neuropeptide release. Furthermore, some nerve endings are large enough to allow the use of the patch-clamp technique. In the present paper we present evidences which show that the isolated neurohypophysial nerve terminals represent a protent tool for studying the mechanism of stimulus-secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01362504
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Release of neuropeptides does not only occur at nerve terminals (1988)
    Springer
    Bioscience reports 8 (1988), S. 471-483 
    Details
    ISSN: 1573-4935
    Keywords: exocytosis ; secretion ; neuropeptides ; vasopressin ; neurohypophysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Neurohypophysial hormones are packed in secretory granules which are stored in nerve endings and in dilatations called nerve swellings. Although it was originally believed that the nerve swellings were storage compartments and that release occurred solely from the nerve terminals, the present paper demonstrates that secretion can occur to the same extent from both nerve endings and nerve swellings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01121646
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural changes in isolated peptidergic nerve terminals induced by digitonin permeabilization and K+ stimulation in the sinus gland of the crab, Cardisoma carnifex (1986)
    Springer
    Cell & tissue research 246 (1986), S. 365-371 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Sinus gland ; Isolated nerve endings ; Potassium stimulation ; Digitonin permeabilization ; Cardisoma carnifex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The preparation of isolated peptidergic nerve terminals from the sinus gland (a neurohemal organ) of the crab (Cardisoma carnifex) is described. In this species the nerve endings can have diameters up to 30 μm. They release neurosecretory material as judged by the decrease in the volumetric density of granules upon depolarization with potassium. Similar results were obtained after permeabilization of the nerve terminals with digitonin, but only in the presence of micromolar concentrations of calcium. This preparation should prove useful in correlating electrical events with other cellular processes involved in stimulus-secretion coupling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215899
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    Paper (German National Licenses)
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