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  • Oxford University Press (OUP)  (3)
  • 1985-1989  (3)
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  • Oxford University Press (OUP)  (3)
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  • 1985-1989  (3)
Year
  • 1
    Online Resource
    Online Resource
    Radioimmunoassay of Deoxynivalenol in Wheat and Corn
    Oxford University Press (OUP) ; 1986
    In:  Journal of AOAC INTERNATIONAL Vol. 69, No. 6 ( 1986-11-01), p. 967-969
    Details
    In: Journal of AOAC INTERNATIONAL, Oxford University Press (OUP), Vol. 69, No. 6 ( 1986-11-01), p. 967-969
    Abstract: With the availability of antibody against deoxynivalenol triacetate (DONtriacetate), a radioimmunoassay (RIA) for DON in wheat was developed. DON is extracted from the sample with acetonitrile-water (84 + 16), defatted with hexane, and then reacted with acetic anhydride to form DON-triacetate. The reaction mixture is loaded onto a C-18 cartridge to remove excess reagents and impurities. Acetylated DON is eluted from the cartridge with 50% methanol in water, and then analyzed by radioimmunoassay utilizing antiserum against DON-triacetate and tritiated DON-triacetate. Overall recovery for DON added to wheat between 50 and 5000 ppb was 86% with a standard deviation of 7% and coefficient of variation of 8%. The limit of detection for DON was about 20 ppb. Analysis of 12 naturally contaminated wheat, corn, and mixed feed samples for DON revealed that RIA results agreed well with thin layer chromatographic analyses performed by other laboratories.
    Type of Medium: Online Resource
    ISSN: 0004-5756
    URL: Article
    DOI: 10.1093/jaoac/69.6.967
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 1986
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Enzyme-Linked Immunosorbent Assay for Deoxynivalenol in Corn and Wheat
    Oxford University Press (OUP) ; 1988
    In:  Journal of AOAC INTERNATIONAL Vol. 71, No. 5 ( 1988-09-01), p. 945-949
    Details
    In: Journal of AOAC INTERNATIONAL, Oxford University Press (OUP), Vol. 71, No. 5 ( 1988-09-01), p. 945-949
    Abstract: The availability of antibody against deoxynivalenol (DON) triacetate (Tri-Ac-DON) has enabled development of a direct enzyme-linked immunosorbent assay (ELISA) and an indirect ELISA for DON in corn and wheat. In both assays, DON is extracted from the sample with acetonitrile- water, reacted with acetic anhydride to form Tri- Ac-DON, and diluted in phosphate buffer for analysis. Direct ELISA was found to be the more sensitive procedure. Fewer interferences are evidenced, and the assay is less time consuming than is indirect ELISA. For direct ELISA, recovery of 10-1000 ppb DON added to corn and wheat was 100% (SD 15, CV 15%) and 102.1% (SD 12.2, CV 11.9%), respectively. For indirect ELISA, overall recovery of 10- 1000 ppb DON added to wheat was 121.5% (SD 39.5, CV 32.5%); in the higher concentration range (500-1000 ppb), recovery was 105% (SD 18, CV 17%). The minimal detection level for DON was around 10 ppb. Analysis of 7 naturally contaminated samples for DON showed that the ELISA results agreed well with those obtained by radioimmunoassay and thin-layer chromatography.
    Type of Medium: Online Resource
    ISSN: 0004-5756
    URL: Article
    DOI: 10.1093/jaoac/71.5.945
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 1988
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    Online Resource
  • 3
    Online Resource
    Online Resource
    Improved Enzyme-Linked Immunosorbent Assay for Aflatoxin Bj in Agricultural Commodities
    Oxford University Press (OUP) ; 1987
    In:  Journal of AOAC INTERNATIONAL Vol. 70, No. 5 ( 1987-09-01), p. 854-857
    Details
    In: Journal of AOAC INTERNATIONAL, Oxford University Press (OUP), Vol. 70, No. 5 ( 1987-09-01), p. 854-857
    Abstract: An improved enzyme-linked immunosorbent assay (ELISA) for aflatoxin B, in cornmeal and peanut butter was developed. Aflatoxin B, in cornmeal and peanut butter samples was extracted with 70% methanol in water containing 1% dimethylformamide diluted with assay buffer to a final concentration of 7.0% methanol, and directly subjected to an ELISA procedure that took less than 1 h for quantitative analysis and less than 30 min for screening tests. Analytical recoveries for 5-100 ppb B, added to the cornmeal and peanut butter were 91 and 95.4%, respectively. The interwell and interassay coefficient of variation was 10% or less at the 20 ppb level and above. Agreement for B, levels in more than 30 naturally contaminated corn, mixed feed, and peanut butter samples was excellent between the ELISA data and the data obtained from different independent laboratories using TLC or other analytical methods
    Type of Medium: Online Resource
    ISSN: 0004-5756
    URL: Article
    DOI: 10.1093/jaoac/70.5.854
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 1987
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