Keywords:
RNA.
;
Molecular biology.
;
Molecular genetics.
;
Electronic books.
Description / Table of Contents:
Molecular Biology of RNA: New Perspectives provides an overview of the developments in RNA research as well as the approaches, strategies, and methodologies used. Most of the contributing authors in the present volume participated in the Fifth Stony Brook Symposium entitled "New Perspectives on the Molecular Biology of RNA" in May 1986. The text is organized into six parts. Part I contains papers dealing with RNA as an enzyme. Part II presents studies on RNA splicing. Part III examines RNA viruses while Part IV focuses on the role of RNA in DNA replication. Part V is devoted to the structure, function, and isolation of RNA. Finally, Part VI takes up the role of RNA in regulation and repression. This volume will help provide new direction and insight for those already working on the subject and will serve as a useful guide to those about to start research in the molecular biology of RNA.
Type of Medium:
Online Resource
Pages:
1 online resource (464 pages)
Edition:
1st ed.
ISBN:
9781483273907
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=1820035
DDC:
574
Language:
English
Note:
Front Cover -- Molecular Biology of RNA: New Perspectives -- Copyright Page -- Table of Contents -- Preface -- PART I: RNA as an Enzyme -- Chapter 1. Cleavage of RNA by RNAse P from Escherichia coli -- I. Introduction -- II. Aspects of the RNase P Reaction -- III. Studies of Enzyme-Substrate Interactions -- IV. Structure-Function Relationships in Ml RNA -- V. Studies of the Protein Subunit of RNase P -- VI. Hybrid Enzymes -- VII. Conclusion -- References -- Chapter 2. Bacillus subtilis RNase P -- I. The RNase P Components -- II. Mechanism of RNase P Cleavage -- III. Toward the Higher-Order Structure of RNase P RNA -- IV. Structure-Function Relationships in the RNase P RNA -- V. Why Is the Catalytic Element of RNase P Composed of RNA? -- References -- Chapter 3. Multiple Enzymatic Activities of an Intervening Sequence RNA from Tetrahymena -- I. Introduction -- II. Self-Splicing RNA -- III. The IVS RNA Enzyme -- References -- Chapter 4. Processing and Genetic Characterization of Self-Splicing RNAs of Bacteriophage T4 -- I. Introduction -- II. Group I Splicing Mechanism for T4 td RNA -- III. Nondirected Mutagenesis and Delineation of Two Functional Domains for Splicing in the td Intron -- IV. Multiple Self-Splicing Introns in T4 -- V. Conclusions -- References -- PART II: RNA Splicing -- Chapter 5. The Mammalian Pre-Messenger RNA Splicing Apparatus: A Ribosome in Pieces? -- I. Introduction -- II. The Discovery of snRNPs -- III. The snRNPs-and-Splicing Hypothesis -- IV. snRNP Components and Structure -- V. Is Eukaryotic RNase P an Sm snRNP? -- VI. Ul snRNPs Bind 5' Splice Sites -- VII. U2, U5, and U4/U6 snRNPs Also Participate in Splicing -- VIII. The Spliceosome-Ribosome Analogy -- References -- Chapter 6. Exon Sequences and Splice Site Proximity Play a Role in Splice Site Selection -- I. Introduction.
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II. Exon Sequences and Splice Site Proximity Play a Role in Splice Site Selection -- III. The Pattern of Splice Site Selection Is Altered in Different Extract Preparations and in Diluted Extracts -- IV. Splice Site Selection Can Be Altered by Competition in Trans -- V. Discussion -- References -- Chapter 7. Factors That Influence Alternative Splice Site Selection in Vitro -- I. Introduction -- II. Materials and Methods -- III. Results -- IV. Discussion -- References -- Chapter 8. Messenger RNA Splicing in Yeast -- I. An Overview of Nuclear mRNA Splicing -- II. Preliminary in Vitro and in Vivo Characterization of Yeast mRNA Splicing -- III. Characterization of Mutations in the Splicing Process -- IV. The RNA Gene Products and the Spliceosome -- V. Speculation -- References -- Chapter 9. Architecture of Fungal Introns: Implications for Spliceosome Assembly -- I. Introduction -- II. Branch Site-3' Splice Junction Relationship -- III. Branch Site-5' Splice Junction Relationship -- IV. Perspectives -- References -- Chapter 10. RNA Joining and Trypanosome Gene Expression -- I. Introduction -- II. Materials and Methods -- III. Results -- IV. Discussion -- V. Summary -- References -- PART III: RNA Viruses -- Chapter 11. The Polio virus Genome: A Unique RNA in Structure, Gene Organization, and Replication -- I. Introduction -- II. Translation and Processing of the Polyprotein -- III. RNA Replication -- IV. Conclusions -- References -- Chapter 12. Permanent Expression of Influenza Virus Genes Coding for Transcriptase Complexes: Complementation of Viral Mutants -- I. Establishment of a Functional Expression System -- II. Addition of Nuclear Protein to the Transcription Complex -- III. Conclusions and Outlook -- References -- Chapter 13. Molecular Mechanisms of Pathogenesis by HTLV-III -- Text -- References -- PART IV: RNA in DNA Replication.
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Chapter 14. Changes in RNA Secondary Structure May Mediate the Regulation of Inc FII Plasmid Gene Expression and DNA Replication -- I. Introduction -- II. RNA Secondary Structure Predictions -- III. Discussion -- References -- Chapter 15. Regulation of Co IE 1 DNA Replication by Antisense RNA -- I. Introduction -- II. RNA Primer Formation -- III. Regulation of Primer Formation -- IV. Binding of RNA I to RNA II -- V. Importance of the Rate of Binding of RNA I to RNA II -- VI. Secondary Structure of RNA II and Its Alterationby Binding of RNA I -- VII. Conclusions -- References -- Chapter 16. A Transfer RNA Implicated in DNA Replication -- I. Introduction -- II. Expression of the dna Y Gene -- III. Implication in DNA Replication -- IV. Summary and Models for Replication Role -- References -- PART V: RNA: Structure, Function, and Isolation -- Chapter 17. Stable Branched RNA Covalently Linked to the 5'End of a Single-Stranded DNA of Myxobacteria -- I. Introduction -- II. DNA Structure of Stigmatella aurantiaca msDNA -- III. RNA Sequence of RNA-Linked msDNA -- IV. Determination of the Linkage between RNA and msDNA -- V. Gene Arrangement of the Coding Regions for msDNA (msd)and msdRNA (msr) on the Chromosome -- VI. Biosynthesis of Branched RNA-Linked msDNA -- VII. How Is Branched RNA-Linked msDNA Synthesized? -- References -- Chapter 18. Recognition of RNA by Proteins -- I. A Simple RNA-Protein Interaction -- II. A Complex RNA-Protein Interaction -- References -- Chapter 19. A New Role for Transfer RNA: A ChloroplastTransfer RNA Is a Cofactor in the Conversionof Glutamate to Delta-Aminolevulinic Acid -- I. Introduction -- II. RNAD A L AIs a Unique Glutamine-Accepting tRNA -- III. RNA as a Cofactor: Possible Reaction Mechanism -- IV. Outlook -- References.
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Chapter 20. Natural Suppressor Transfer RNA in Eukaryotes: Its Implication in the Evolution of the Genetic Code and Expression of Specific Genes -- I. Introduction -- II. Isolation from Tetrahymena of the tRNA Gene and tRNA Corresponding to the Termination Codon UAA -- III. Deviation of the Genetic Code of Tetrahymena from the Universal Genetic Code -- IV. Evolution of Glutamine tRNAs Recognizing UAA and UAG Termination Codons in Tetrahymena -- V. Isolation of a Natural UAG Suppressor Glutamine tRNA from Mouse Cells -- VI. A Large Increase of tRNAg [ -- UG in Mouse Cells Infected withMo-MuLV -- VII. Concluding Remarks -- References -- Chapter 21. The Purification of Small RNAs by High-Performance Liquid Chromatography -- I. Introduction -- II. Materials and Methods -- III. Results -- IV. Discussion -- References -- Chapter 22. Comparative Studies on the Secondary Structure of the RNAs of Related RNA Coliphages -- I. Introduction -- II. Results -- III. Discussion -- References -- VI RNA in Regulation and Repression -- Chapter 23. Autogenous Regulation of Transcriptionof the crp Operon by a Divergent RNA Transcript -- I. Introduction -- II. Activation of a Divergent Promoter by Cyclic AMP-CRP Is Required for crp Autoregulation -- III. Autoregulation of crp Is Mediated by Divergent RNA -- IV. A Model for crp Autoregulation -- V. Conclusion -- References -- Chapter 24. The Role of Translational Regulationin Growth Rate-Dependent and Stringent Control of the Synthesis of Ribosomal Proteinsin Escherichia col -- I. Secondary Structure of the LI Target Site on Lll mRNA -- II. Growth Rate-Dependent Control of Ribosomal Protein Synthesis -- III. Stringent Control of Ribosomal Protein Synthesis -- IV. Concluding Remarks -- References -- Chapter 25. Sequence and Structural Elements Associatedwith the Degradation of Apolipoprotein II Messenger RNA.
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I. Introduction -- II. Effect of 5' Noncoding Sequences on apo II mRNA Stability -- III. Use of Enzymatic Probes and Reverse Transcriptase to Analyze apo II mRNA Secondary Structure -- IV. Analysis of apo II mRNA in Polysomes -- V. Summary and Perspective -- References -- Chapter 26. A New Immune System against Viral Infection Using Antisense RNA: micRNA-Immune System -- I. Introduction -- II. micRNA Mutagenesis -- III. micRNA-Immune System -- IV. Conclusion -- References -- Chapter 27. Regulation of IS10 Transposase Expression by RNA/RNA Pairing -- I. Introduction -- II. Biological Role of Multicopy Inhibition -- III. Molecular Mechanism of Multicopy Inhibition -- IV. Mechanism of Pairing between RNA-INand RNA-OUT in Vitro -- V. In Vivo Phenotypes of Mutations in RNA-OUT -- References -- Chapter 28. Characterization and Functional Analysis of theFactors Required for Transcription of the Adenovirus Major Late Promoter -- I. Introduction -- II. Fractionation and Functional Analysis of the Factors Required for Transcription of the Adenovirus Major Late Promoter -- III. Concluding Remarks -- References -- Index.
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