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Macrophages in normal human bone marrow and in chronic myeloproliferative disorders: An immunohistochemical and morphometric study by a new monoclonal antibody (PG-M1) on trephine biopsies (1992)

Thiele, Juergen ; Braeckel, Christiane ; Wagner, Stephan ; [et al.]

Springer

Virchows Archiv 421 (1992), S. 33-39

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ISSN: 1432-2307

Keywords: Macrophages ; Haemosiderin ; Osteoclasts ; Monoclonal antibody PG-M1 ; Bone marrow

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary An immunohistochemical and morphometric study was performed on routinely processed trephine biopsies of the bone marrow in 30 normal individuals and in 90 patients with various subtypes of chronic myeloproliferative disorder. Using a new monoclonal antibody (PG-M1) directed against a formalin-resistant epitope on macrophages and by employment of the Prussian blue reaction, quantitation of this cell population was feasible. Morphometric analysis revealed that the number of iron-laden macrophages represented only a fraction of the total number of histiocytic reticular cells. As could be expected, in polycythaemia rubra vera, no haemosiderin deposits were detectable, but the content of macrophages slightly exceeded that of the normal bone marrow. In chronic myeloid leukaemia 9 of 30 patients showed a significant increase in PG-M1-positive reticular cell elements. These were consistent with pseudo-Gaucher cells, sea-blue histiocytes and intermediate cell types. Primary (idiopathic) myelofibrosis-osteomyelosclerosis was characterized by a significant increase in macrophages (25 of 30 patients). Involvement of macrophages in the complex mechanisms generating bone marrow fibrosis and angiogenesis and in bone remodelling (osteosclerosis) may be responsible for this finding.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01607136

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Paper (German National Licenses)

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Synthese von ( - )-(Acetoxymethyl)(hydroxymethyl)methyl(phenyl)german [( - )-MePhGe(CH2OAc)(CH2OH)] durch eine Esterase-katalysierte Umesterung: Die erste enzymatische Synthese eines optisch aktiven Germans (1994)

Tacke, Reinhold ; Wagner, Stephan A. ; Sperlich, Jörg

Weinheim : Wiley-Blackwell

Berichte der deutschen chemischen Gesellschaft 127 (1994), S. 639-642

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ISSN: 0009-2940

Keywords: Germane, optically active ; Biotransformation, stereoselective ; Transesterification, enzymatic ; Porcine liver esterase ; Chemistry ; Inorganic Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: Synthesis of ( - )-(Acetoxymethyl)(hydroxymethyl)methyl(phenyl)germane [( - )-MePhGe(CH2OAc)(CH2OH)] by an Esterase-Catalyzed Transesterification: the First Enzymatic Synthesis of an Optically Active GermaneThe prochiral germane MePhGe(CH2OH)2 (1) was synthesized by a six-step synthesis starting from GeCl4 (3) [3 → Cl2Ge(CH2Cl)2 (4) → Ph2Ge(CH2Cl)2 (5) → (CF3S(O)2O)PhGe(CH2Cl)2 (6) → MePhGe(CH2Cl)2 (7) → MePhGe(CH2OAc)2 (8) → 1]. Reaction of 1 with Ac2O/NEt3 (molar ratio 1: Ac2O = 1:1) gave the racemic germane rac-MePhGe(CH2OAc)(CH2OH) (rac-2). Stereoselective transesterification of 1 with vinyl acetate (acetate source and solvent), catalyzed by immobilized porcine liver esterase (PLE; E.C.-3.1.1.1), yielded the optically active germane ( - )-MePhGe(CH2OAc)(CH2OH) [( - )-2] (yield 57%, enantiomeric purity 50% ee).