ISSN:
1432-072X
Keywords:
Acetobacterium woodii
;
Fructose growth yields
;
Chemostat culture
;
Yeast extract
;
Carbon dioxide
;
YATP
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The growth of the anaerobic acetogenic bacterium Acetobacterium woodii DSM 1030 was investigated in fructose-limited chemostat cultures. A defined medium was developed which contained fructose, mineral salts, cysteine · HCl and Ca pantothenate (1 mg · 1−1) supplied in a vitamin supplement. Growth at high dilution rates was dependent on the presence of CO2 in the gas phase. The μ max was found to be 0.16 h−1 and the fructose maintenance requirement was 0.1 to 0.13 mmol fructose · (g dry wt)−1 · h−1. A growth yield of 61 g dry wt · (mol fructose)−1, corrected for the cell maintenance requirement and for incorporation of fructose carbon into cell biomass, was determined from the fructose consumption. A corresponding growth yield of 69 g dry wt · (mol fructose)−1 was calculated from the acetate production assuming that fructose fermentation was homoacetogenic. A YATP of 12.2 to 13.8 g dry wt · (mol ATP)−1 was calculated from these growth yields using a value of 5 mol ATP · (mol fructose)−1 as an estimate of the amount of ATP synthesised from fructose fermentation. The addition of yeast extract (0.5 g · 1−1) to the medium did not influence the μ max or cell yield. After prolonged growth under fructose-limited conditions the requirement of the culture for CO2 in the gas phase was reduced.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00249170
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