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  • 1
    Electronic Resource
    Electronic Resource
    Wissenschaft (1994)
    Weinheim : Wiley-Blackwell
    Biologie in unserer Zeit 24 (1994), S. 244-245 
    Details
    ISSN: 0045-205X
    Keywords: Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/biuz.19940240505
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  • 2
    Electronic Resource
    Electronic Resource
    Between DNA and protein – RNA editing in plant mitochondria (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 81 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Higher plants contain the largest mitochondrial genomes found so far. Several factors contribute to this expansion in size, notably integrated plastid and nuclear sequences; numerous repeats, some of which are active in recombination and sequence rearrangements; introns of more than 3 400 nucleotides and several genes unique to plant mitochondrial DNA. Genes are transcribed into mono and polycistronic mRNAs and translated by the standard genetic code. However, mRNAs are altered from the DNA encoded sequence by RNA editing with mostly cytidine to uridine and occasionally uridine to cytidine transitions. Edited mRNAs specify different polypeptides than those predicted by open reading frames in the DNA. Partially edited mRNA molecules raise the question of which proteins are actually synthesized. RNA editing of mitochondrial transcripts appears to occur in all higher plants and may date back to the common ancestors of modern plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb08754.x
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  • 3
    Electronic Resource
    Electronic Resource
    Cytochrome oxidase subunit II mRNAs in Oenothera mitochondria are edited at 24 sites (1990)
    Springer
    Current genetics 18 (1990), S. 371-375 
    Details
    ISSN: 1432-0983
    Keywords: Plant mitochondria ; RNA editing ; Cytochrome oxidase subunit II ; Partial editing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Analysis of cDNA clones covering the entire coding region of cytochrome oxidase subunit II in Oenothera mitochondria reveals 24 potential editing sites: 23 C to U transitions and one U to C conversion. One editing event is observed outside the open reading frame in the 3′ non-coding region. Thirteen editing sites are found altered in all cDNA clones, whereas the other eleven sites are only edited in some of the cDNA clones. These differentially edited sites occur most frequently at silent codon positions or in triplets at non-conserved amino acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318219
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  • 4
    Electronic Resource
    Electronic Resource
    Genes for tRNAGly, tRNAHis, tRNALys, tRNAPhe, tRNASer and tRNATyr are encoded in Oenothera mitochondrial DNA (1990)
    Springer
    Current genetics 17 (1990), S. 353-358 
    Details
    ISSN: 1432-0983
    Keywords: Plant mitochondria ; Oenothera ; tRNA genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genes coding for tRNAGly, tRNAHis, tRNALys, tRNAPhe, tRNASer and tRNATyr have been identified in Oenothera mitochondrial DNA. Sequence analysis of these genes and their surrounding sequences are presented and compared with other known tRNA genes from plant mitochondria. All six deduced tRNA sequences can be folded into the classical cloverleaf structure model. Only the tRNAHis gene shows high homology with the corresponding chloroplast gene and thus appears to be derived from a transfer event of chloroplast sequences into the mitochondrial genome. The sequences surrounding this gene, however, show little similarity with the chloroplast genome. The other five deduced tRNAs display a much lower similarity with their chloroplast counterparts and thus appear to be genuine mitochondrial tRNAs. These tRNAs are highly conserved between monocots and dicots with maximally three nucleotides differing between the Oenothera sequences and their wheat homologues. A purine-rich sequence is found upstream of each tRNA gene in Oenothera, similar to wheat mitochondrial tRNA genes, that could be involved in transcription signalling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00314884
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  • 5
    Electronic Resource
    Electronic Resource
    An rps14 pseudogene is transcribed and edited in Arabidopsis mitochondria (1993)
    Springer
    Current genetics 24 (1993), S. 330-336 
    Details
    ISSN: 1432-0983
    Keywords: Arabidopsis mitochondria ; rpl5/pseudo-rps14/cob ; RNA editing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sequence analysis of the region upstream of the apocytochrome b (cob) gene in the Arabidopsis mitochondrial genome identifies an open reading frame with homology to ribosomal protein L5, (rpl5), and a pseudogene with similarity to ribosomal protein S14 (rps14) genes. Both cob and rpl5 genes have intact reading frames, but the rps14 homology is disrupted by a stop codon and a deleted nucleotide. The rpl5 gene, the rps14 pseudogene, and the cob gene are separated by one nucleotide and a 1604-nucleotide-long spacer respectively. A plastid-like tRNASer is encoded downstream from the cob gene. The entire region is transcribed into a 5-kb transcript, containing the rps14 pseudogene and the cob gene. Cob and rpl5 mRNAs are edited in several positions with different frequencies. The rps14 pseudogene is transcribed and edited in one position in common with other plants. Since no intact rps14 gene is found in the mitochondrial genome of Arabidopsis, the functional gene is presumably encoded in the nucleus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00336785
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  • 6
    Electronic Resource
    Electronic Resource
    Distribution of RNA editing sites in Oenothera mitochondrial mRNAs and rRNAs (1991)
    Springer
    Current genetics 20 (1991), S. 397-404 
    Details
    ISSN: 1432-0983
    Keywords: RNA editing ; rRNAs ; atpA ; cytb ; orfB ; Plant mitochondria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To investigate whether RNA editing in plant mitochondria modifies structural RNAs as well as protein-coding RNAs we compared the genomic-encoded information with the respective transcripts of several genes in Oenothera. The genes analysed are the 5S, 18S and 26 S rRNAs, the alpha-subunit of ATPase (atpA), cytochrome b (cytb), orfB, which is located upstream of cytochrome oxidase subunit III, and the respective leader, trailer and spacer sequences. All open reading frames were found to be edited to some degree. The atpA coding region has the least edited mRNA in Oenothera mitochondria, with only four nucleotides altered in the 1533 nucleotide open reading frame. From this analysis we conclude that frequent RNA editing is indicative of functional protein coding regions in plant mitochondria. The extensive editing in orfB, for example, suggests that this orf codes for a mitochondrial protein. No RNA editing event was found in the 5S rRNA or in the 1824 nucleotides analysed of the 18S rRNA, but two nucleotides were found to be altered in the 1970 nucleotides compared for the 26S rRNA. One nucleotide alteration has changed C to U, the other in reverse U to C. However, only one of five cDNA clones covering this region shows the modifications, similar to many silent editing events in open reading frames. RNA editing in the structural RNAs thus does not seem to be essential for their function in the mitochondrial ribosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317068
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  • 7
    Electronic Resource
    Electronic Resource
    A mitochondrial intron sequence in the 5′-flanking region of a plant nuclear lectin gene (1991)
    Springer
    Current genetics 20 (1991), S. 423-425 
    Details
    ISSN: 1432-0983
    Keywords: Endosymbiont theory ; Mitochondrial intron ; Lectin genes ; Sequence transfer ; Plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A sequence fragment from the cis-splicing intron between exons a and b of the NADH-dehydrogenase subunit 5 gene (nad5) in plant mitochondria is also present in one of two closely related nuclear-encoded lectin genes of Dolichos biflorus. This sequence of 116 nucleotides is the major difference in the 5′-flanking region of two recently described lectin genes (Harada et al. 1990). The stem and leaf lectin DB58 does not contain the insert, while the otherwise more than 90% identical 5′-flanking region of the seed lectin is interrupted by this mitochondrial intron sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317072
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  • 8
    Electronic Resource
    Electronic Resource
    Transcription of potato mitochondrial 26S rRNA is initiated at its mature 5′ end (1994)
    Springer
    Current genetics 26 (1994), S. 519-523 
    Details
    ISSN: 1432-0983
    Keywords: Potato ; Plant mitochondria ; 26S rRNA ; Invitro capping ; Mitochondrial promoters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transcription initiation sites in plant mitochondria can be located by in vitro capping of primary 5′ transcript termini. Direct sequencing of a cap-labelled mitochondrial RNA from potato shows its sequence to be identical to the 5′ terminal part of the 26S rRNA. Primer extension analysis indicates the mature 5′ end to be the sole detectable 5′ transcript terminus. In potato mitochondria the mature 5′ end of the 26S rRNA is thus created by transcription initiation without any further 5′ processing. The nucleotide sequence surrounding this transcription initiation site shows only limited similarity to other putative promoter sequences from dicot plant mitochondria suggesting the possibility that divergent RNA polymerases, and/or transcription initiation factors, are present in plant mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00309943
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  • 9
    Electronic Resource
    Electronic Resource
    Rps3 and rpl16 genes do not overlap in Oenothera mitochondria: GTG as a potential translation initiation codon in plant mitochondria? (1994)
    Springer
    Plant molecular biology 24 (1994), S. 811-818 
    Details
    ISSN: 1573-5028
    Keywords: group II intron ; recombination ; RNA editing ; rpl16 ; rps3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Characterization of the Oenothera mitochondrial ribosomal gene cluster rps19-rps3-rpl16 shows the two genes rps3 and rpl16 to be separated by 9 nucleotides. The first codon of rpl16 is a GTG codon for valine and the only potential translational start. This GTG codon is conserved at the same position in maize, Petunia and Marchantia mitochondria, while sequences diverge upstream. These observations suggest that GTG at least at this position may act as translation initiation codon in plant mitochondria. Analysis of RNA editing suggests both genes to code for functional ribosomal proteins in Oenothera mitochondria. A duplication/recombination event at a decanucleotide in the intron of rps3 created a pseudogene missing part of the intron and the 3′ exon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029863
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  • 10
    Electronic Resource
    Electronic Resource
    RNA editing gives a new meaning to the genetic information in mitochondria and chloroplasts (1993)
    Springer
    Plant molecular biology 21 (1993), S. 1163-1170 
    Details
    ISSN: 1573-5028
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RNA editing in plant mitochondria and chloroplasts alters mRNA sequences to code for different proteins than the DNA. Most of these C-to-U transitions occur in open reading frames, but a few are observed in intron sequences. Influences of the nuclear genome on editing patterns suggest that cytoplasmic factors participate in this process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023611
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