In:
Journal of Veterinary Diagnostic Investigation, SAGE Publications, Vol. 2, No. 4 ( 1990-10), p. 334-337
Abstract:
A Mycoplasma iowae (MI) species-specific DNA probe (designated pMI-2) of 6.0 kbp (kilobase pairs) was isolated from an MI strain I-695 genomic library prepared in plasmid pUC8 and Escherichia coli strain JM83. When labeled with [32] P by nick translation, the probe hybridized in dot blot assays with 6 reference strains and 8 field isolates of MI but not with 16 other known species of avian mycoplasmas. The pMI-2 probe detected a minimum of 1.5 ng of MI strain I-695 chromosomal DNA. Under identical conditions of hybridization, the probe did not hybridize with a high concentration (200 ng) of M. gallisepticum or M. synoviae chromosomal DNA.
Type of Medium:
Online Resource
ISSN:
1040-6387
,
1943-4936
DOI:
10.1177/104063879000200415
Language:
English
Publisher:
SAGE Publications
Publication Date:
1990
detail.hit.zdb_id:
2265211-5
SSG:
22
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