In:
European Journal of Biochemistry, Wiley, Vol. 219, No. 1-2 ( 1994-01), p. 555-562
Abstract:
We have isolated and characterized cDNA and genomic DNA clones encoding the catalytic subunit (C) of cAMP‐dependent protein kinase in the aquatic fungus Blastocladiella emersonii . The C‐subunit amino acid sequence derived from the nucleotide sequence predicts a basic polypeptide of 424 residues, excluding the initiator methionine, which by amino‐terminal sequence analysis has been shown to be absent from the mature protein. The Blastocladiella C presents a 70‐amino‐acid extension at the amino terminus, when aligned to the mouse C α subunit, being one of the largest C subunits already characterized. The B. emersonii C‐gene‐coding region is interrupted by three introns, ranging in size over 57–69 bp. The positions of the introns are quite different from those found in other species, suggesting a considerable amount of evolutionary drift in the gene structure. The 5′‐flanking region lacks recognizable TATA or CCAAT sequences, is remarkably high in GC content (70%), and primer extension experiments indicate that transcription initiates from multiple sites. Several sequence motifs were identified in the promoter region which could be involved in the developmental control of this gene.
Type of Medium:
Online Resource
ISSN:
0014-2956
,
1432-1033
DOI:
10.1111/ejb.1994.219.issue-1-2
DOI:
10.1111/j.1432-1033.1994.tb19971.x
Language:
English
Publisher:
Wiley
Publication Date:
1994
detail.hit.zdb_id:
1398347-7
detail.hit.zdb_id:
2172518-4
SSG:
12
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