In:
Proceedings, annual meeting, Electron Microscopy Society of America, Cambridge University Press (CUP), Vol. 48, No. 3 ( 1990-08-12), p. 580-581
Abstract:
Envelope is a very important structure for viral attachment and entry into the host cell, but it is also a morphologically variable portion of enveloped viruses. Studying the fine structure of enveloped viruses, we noticed that different sample preparations of viruses resulted in the change of viral size and shape to some extent, which we believe was caused by the variation of the viral envelope. Four typical enveloped viruses: IBRV (Infectious Bovine Rhinotracheitis Virus), GPV (Goat Pox Virus), SbV (Sindbis Virus) and VSV (Vesicular Stomatitis Virus) were investigated in our experiments. Host cells infected with IBRV, GPV, SbV and VSV respectively were fixed with 1-5% glutaraldehyde in Hank's buffer when the cytopathic effects appeared in 50-70% of the cells, then the specimens were treated respectively with different conventional methods of EM sample preparation: 1) ultrathin sectioning, 2) negative staining,3) freeze etching, 4) surface replica, 5) whole mount or SEM observations. All the samples were examined under JEM-200CX TEM or JSM-35CF SEM.
Type of Medium:
Online Resource
ISSN:
0424-8201
,
2690-1315
DOI:
10.1017/S0424820100160455
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
1990
SSG:
11
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