In:
FEBS Letters, Wiley, Vol. 343, No. 3 ( 1994-05-02), p. 251-255
Abstract:
Annexin‐I is a calcium dependent phospholipid binding and phospholipase A 2 (PLA 2 ) inhibitory protein. A ‘substrate depletion’ model has been proposed for the mechanism of PLA 2 inhibition by annexin‐I in studies with 14 to 18 kDa PLA 2 s. Herein, we have studied the inhibition mechanism using 100 kDa cytosolic PLA 2 from porcine spleen. The inhibition has been measured at various substrate and calcium ion concentrations. The pattern of PLA 2 inhibition by annexin‐I was consistent with a ‘specific interaction’ mechanism rather than the ‘substrate depletion’ model. Apparent contradiction with previous studies can be explained by the calcium‐dependent binding of annexin‐I to the substrate.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(94)80566-0
Language:
English
Publisher:
Wiley
Publication Date:
1994
detail.hit.zdb_id:
1460391-3
SSG:
12
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