In:
Journal of Clinical Microbiology, American Society for Microbiology, Vol. 29, No. 3 ( 1991-03), p. 470-474
Abstract:
Nasopharyngeal secretions obtained from 94 children with acute respiratory illness were examined for the presence of respiratory syncytial virus (RSV), adenovirus, and influenza virus type A by virus culturing (virus isolation technique [VIT] ), immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA). Similar results were obtained in at least two tests for RSV, influenza virus type A, and adenovirus in 92 (97.9%), 88 (93.6%), and 88 (93.6%) cases, respectively. Both rapid virus detection methods showed good specificity for the diagnosis of these virus infections (greater than or equal to 90.7%) and were more sensitive than was VIT for RSV detection. In a more accurate statistical analysis, the indexes of agreement between VIT and ELISA were substantial for RSV (kappa = 0.69; zeta = 5.5; P less than 0.0001), influenza virus type A (kappa = 0.67; zeta = 5.3; P less than 0.0001), and adenovirus (kappa = 0.71; zeta = 6.0; P less than 0.0001), while it was almost perfect for RSV when ELISA was compared with IFA (kappa = 0.88; zeta = 5.7; P less than 0.0001). Although the observed agreement was good in the comparison of these two tests for these three viruses (89%0, the indexes of agreement were moderate in the comparison of IFA and VIT for RSV (K = 0.55; Z = 2.0; P 〈 0.05), influenza virus type A (K = 0.42; Z = 9.7; P 〈 0.0001), and adenovirus (K = 0.41; Z = 6.5; P 〈 0.0001) and of ELISA and IFA for influenza virus type A (K = 0.55; Z = 7.0; P 〈 0.0001) and adenovirus (K = 0.59; Z = 6.8; P 〈 0.0001). All of the statistical evaluations demonstrated better agreement between ELISA and VIT for influenza virus type A and adenovirus.
Type of Medium:
Online Resource
ISSN:
0095-1137
,
1098-660X
DOI:
10.1128/jcm.29.3.470-474.1991
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1991
detail.hit.zdb_id:
1498353-9
SSG:
12
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