In:
FEBS Letters, Wiley, Vol. 326, No. 1-3 ( 1993-07), p. 246-250
Abstract:
An antibody against the NDH‐K subunit of the NAD(P)H‐dehydrogenase from the cyanobacterium Synechocystis sp. PCC6803 was used to isolate a subcomplex of the enzyme from Triton X‐100 solubilized total membranes by immunoaffinity chromatography. The isolated subcomplex consisted of seven major polypeptides with molecular masses of 43, 27, 24, 21, 18, 14 and 7 kDa. The amino‐terminal amino acid sequences of the polypeptides were determined. By comparing the sequences with the amino acid sequences deduced from DNA, three proteins were identified as NDH‐H (43 kDa), NDH‐K (27 kDa) and NDH‐I (24 kDa). A fourth subunit (NDH‐J, 21 kDa) was identified by Western blot analysis with an NDH‐J antibody.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(93)81800-F
Language:
English
Publisher:
Wiley
Publication Date:
1993
detail.hit.zdb_id:
1460391-3
SSG:
12
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