ISSN:
1573-8221
Keywords:
human insulin gene
;
polymerase chain reaction
;
subcloning of the insulin gene
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract A method for cloning the insulin gene from total human DNA is developed on the basis of the polymerase chain reaction. Cloning of the insulin gene from the total DNA of human lymphocytes is performed to obtain a pNP-90 vector for subsequent expression of the insulin gene in nonendocrine cells for the purposes of gene therapy of diabetic angiopathies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02446480
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