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  • Life and Medical Sciences  (1)
  • Tryptophan metabolism  (1)
  • 1990-1994  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Repression of toxin production by tryptophan in Clostridium botulinum type E (1990)
    Springer
    Archives of microbiology 154 (1990), S. 443-447 
    Details
    ISSN: 1432-072X
    Keywords: Clostridium botulinum type E ; Tryptophan metabolism ; Nitrogen repression ; Botulinum neurotoxin formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Of the seven amino acids required by Clostridium botulinum type E, tryptophan is the most essential and may provide the cell with nitrogen. The addition of excess tryptophan (10–20 mM) or other nitrogenous nutrients to minimal growth medium markedly decreased toxin formation but did not affect growth in C. botulinum type E. On the other hand, the addition of an enzymatic digest of casein (NZ Case) stimulated toxin formation and overcame repression by tryptophan. Immunoblots of proteins in culture fluids using antibodies to type E toxin indicated that tryptophan-repressed cultures produced less neurotoxin protein. Inhibitors of neurotoxin did not accumulate in cultures grown in minimal medium supplemented with high tryptophan. The results suggest that tryptophan availability in foods or in the intestine may be important for toxin formation by C. botulinum type E.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00245225
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Epidermis generated in vitro: practical considerations and applications (1991)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 45 (1991), S. 245-251 
    Details
    ISSN: 0730-2312
    Keywords: epidermis ; skin ; skin graft ; cell culture ; in vitro ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The technology for culture of epidermis is one of the most advanced to date for generation of a tissue in vitro. Cultured epidermis is already used for a number of applications ranging from use as a permanent skin replacement to use as an organotypic culture model for toxicity testing and basic research. While simple epidermal sheets have been grafted successfully, more advanced models for skin replacement consisting of both dermal and epidermal components are in development and being tested in a number of laboratories. One of the most advanced in vitro models is the living skin equivalent, an organotypic model consisting of a collagen lattice contracted and nourished by dermal fibroblasts overlaid with a fully formed epidermis.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240450304
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    Paper (German National Licenses)
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