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Scanning and transmission electron-microscopic study of peripolar cells in the newborn lamb kidney (1993)

Thumwood, Cassandra M. ; McCausland, Jane ; Alcorn, Daine ; [et al.]

Springer

Cell & tissue research 274 (1993), S. 597-604

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ISSN: 1432-0878

Keywords: Peripolar cells ; Juxtaglomerular apparatus ; Cytoplasmic granules ; Exocytosis ; Electron microscopy ; Sheep, newborn

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract Scanning and transmission electron microscopy were used to study the ultrastructural characteristics and positions of granulated peripolar cells in newborn lamb kidney. Following tissue fixation by vascular perfusion in situ, the vascular pole region of the glomerulus was exposed for examination by scanning electron micoscopy following removal of the glomerular tuft. Peripolar cells were recognized by their surface morphology enabling their quantification and an assessment of the relationship of their position in the renal cortex. The prominent expression of peripolar cells in this species was confirmed. Almost every vascular pole examined revealed peripolar cells (405 out of 407; 99.5%) and thus, throughout the cortex, the distribution of peripolar cells was the same as the distribution of renal corpuscles. Larger, more protruding peripolar cells were observed in the outer cortical renal corpuscles. The numbers of peripolar cells encircling each vascular pole ranged from 1 to 10. There was no correlation between number of granulated peripolar cells at the vascular pole and the position of the renal corpuscle within the renal cortex. As viewed by transmission electron microscopy, organelles of protein synthesis were abundant in the cytoplasm of peripolar cells. Exocytosis of cytoplasmic granules was observed by both scanning and transmission electron microscopy implying that a process of regulative secretion occurs from these cells. The use of ultrastrural techniques has provided evidence supporting the concept that peripolar cells are prominent in the cuff region of each renal corpuscle of the newborn lamb and further-more that peripolar cells in this species most likely have a secretory function.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00314558

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Immunohistochemical studies of renin-containing cells in the developing sheep kidney (1994)

Kon, Yasuhiro ; Alcorn, Daine ; Murakami, Kazuo ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

The @Anatomical Record 239 (1994), S. 191-197

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ISSN: 0003-276X

Keywords: Development ; Immunohistochemistry ; Renin-containing cells ; Sheep ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine

Notes: Background: Renin-containing (RC) cells in small ruminant kidneys have been known to be widely distributed along the blood vessels. In the present study, RC cells in developing sheep kidneys were studied to investigate not only the appearance but distribution with the potential physiological significance using immunohistochemical and histophanimetrical techniques.Methods: Seven fetal, 12 newborn, and 3 adult metanephric kidneys were used and immunostained by anti-renin antiserum. In the histoplanimetrical analysis, the numerical values of RC cells existing at the walls of 3 major arterial types in the kidneys were calculated.Results: At day 44 of gestation, RC cells were already demonstrated in the walls of renal, interlobar, and afferent vessels, located in the deep cortex and the medulla. In intermediate gestational periods, RC cells were detected throughout the intrarenal arterial trees. In late gestational periods, RC cells expressed in the walls of interlobar/arcuate and interlobular arteries tended to decrease or disappear gradually, while they were distributed predominantly in the afferent glomerular vessels. In newborn lambs, especially days 1 to 3 after birth, increased numbers of RC cells were demonstrated throughout the arterial trees in the kidneys. In older lambs, RC cells located in the interlobar/arcuate arteries and the proximal region of the interlobular arteries decreased in number and gradually disappeared. Some RC cells were still distributed in the distal portion of the interlobular artery even in the adult sheep.Conclusions: These results suggest that the wide distribution of RC cells in sheep kidney is formed in perinatal life, and that the neuronal regulation is associated with the maintenance of this distribution. © 1994 Wiley-Liss, Inc.

Additional Material: 8 Ill.