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Qualitative and quantitative analysis of granules in atrial appendage cardiocytes in different physiological states (1990)

Gall, John A. M. ; Alcorn, Daine ; Fernley, Ross ; [et al.]

Springer

Cell & tissue research 259 (1990), S. 529-534

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ISSN: 1432-0878

Keywords: Atrial appendage ; Atrial-specific granules ; Atrial natriuretic polypeptides ; Exocytosis ; Ultrastructure ; Rat (Sprague-Dawley)

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary Atrial appendage cardiocytes of mammals, including man, contain multiple cytoplasmic granules that vary in number in different physiological states. Using morphologic and comprehensive morphometric techniques, these granules were assessed in Sprague-Dawley rats following dehydration for 5 days, volume-loading by substituting 1% NaCl as drinking water for 7 days, unilateral nephrectomy plus volume-loading for 7 days, and in late term pregnant animals (18–20 days; term ≈21 days). Although principally located in the paranuclear region, granules were observed throughout the sarcoplasm. Cytological features indicative of synthetic activity and granule formation were readily apparent in all groups with the exception of pregnant rats where they were infrequently observed. Granule contents were released by exocytosis and observed in the right appendage of control, dehydrated and nephrectomy/volume-loaded groups and left appendage of volumeloaded animals. Exocytosis was not observed in pregnant animals. By point counting, the proportional volume of cardiocytes occupied by granules (V v ) in controls was significantly greater for right than for left appendage (2.12±0.22% vs 1.29±0.16%; mean±SEM;p〈0.05). A significantly similar difference was found for nephrectomy/volume-loaded animals. There was no significant difference inV v for right appendage between the control and experimental groups; for left appendage there was a significant increase inV v to 2.42±0.09% (p〈0.05) for volume-loaded animals only. Estimation of the maximum diameter of granule profiles in control animals was 238±9 nm and 230±6 nm for right and left appendages, respectively. The profile diameters in the left appendages of dehydrated (202±9 nm) and pregnant (200±7 nm) animals were significantly (p〈0.05) less than those of the control animals. The morphometric findings did not correlate with predictions based upon published biochemical data. In the course of this study, a previously unreported bimembranous, circular to ovoid structure was observed in the cardiocyte sarcoplasm of all animals; the nature and function of this structure is unknown.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01740780

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Immunohistochemical studies of renin-containing cells in the developing sheep kidney (1994)

Kon, Yasuhiro ; Alcorn, Daine ; Murakami, Kazuo ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

The @Anatomical Record 239 (1994), S. 191-197

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ISSN: 0003-276X

Keywords: Development ; Immunohistochemistry ; Renin-containing cells ; Sheep ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine

Notes: Background: Renin-containing (RC) cells in small ruminant kidneys have been known to be widely distributed along the blood vessels. In the present study, RC cells in developing sheep kidneys were studied to investigate not only the appearance but distribution with the potential physiological significance using immunohistochemical and histophanimetrical techniques.Methods: Seven fetal, 12 newborn, and 3 adult metanephric kidneys were used and immunostained by anti-renin antiserum. In the histoplanimetrical analysis, the numerical values of RC cells existing at the walls of 3 major arterial types in the kidneys were calculated.Results: At day 44 of gestation, RC cells were already demonstrated in the walls of renal, interlobar, and afferent vessels, located in the deep cortex and the medulla. In intermediate gestational periods, RC cells were detected throughout the intrarenal arterial trees. In late gestational periods, RC cells expressed in the walls of interlobar/arcuate and interlobular arteries tended to decrease or disappear gradually, while they were distributed predominantly in the afferent glomerular vessels. In newborn lambs, especially days 1 to 3 after birth, increased numbers of RC cells were demonstrated throughout the arterial trees in the kidneys. In older lambs, RC cells located in the interlobar/arcuate arteries and the proximal region of the interlobular arteries decreased in number and gradually disappeared. Some RC cells were still distributed in the distal portion of the interlobular artery even in the adult sheep.Conclusions: These results suggest that the wide distribution of RC cells in sheep kidney is formed in perinatal life, and that the neuronal regulation is associated with the maintenance of this distribution. © 1994 Wiley-Liss, Inc.

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