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  • Springer  (3)
  • 1990-1994  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Cytoplasmic male sterility is associated with large deletions in the mitochondrial DNA of two Nicotiana sylvestris protoclones (1992)
    Springer
    Current genetics 21 (1992), S. 131-137 
    Details
    ISSN: 1432-0983
    Keywords: mtDNA ; Recombination ; CMS ; N. sylvestris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two cytoplasmic male-sterile plants (CMSI and CMSII) were obtained by protoplast culture in Nicotiana sylvestris. Both plants showed large deletions (up to 50 kb) in their mitochondrial DNA. Restriction maps of the reorganized regions suggested that the deletions occurred via two homologous recombination events (rec. 1 and rec. 2) in the parental mitochondrial genome. With the exception of nad5, no mitochondrial DNA polymorphism could be detected between parental and CMS lines using different heterologous genes probes. A sequence homologous to the Oenothera nad5 mitochondrial gene was located close to the CMSI-specific rec. 2 region. Moreover, a cDNA probe corresponding to total mitochondrial RNA from the parent line was found to hybridize to mitochondrial DNA fragments involved in the rec. 1 event common to both CMS lines, suggesting that rec. 1 lies in a transcribed region. Cytoplasmic male sterility in the Nicotiana sylvestris CMS mutants could be due either to gene deletion or to a regulatory effect of such a deletion on mitochondrial gene expression, rather than to the presence of specific polypeptides as has been shown in the T cytoplasm of maize, or in CMS Petunia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318472
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Several nuclear genes control both male sterility and mitochondrial protein synthesis inNicotiana sylvestris protoclones (1990)
    Springer
    Molecular genetics and genomics 222 (1990), S. 206-210 
    Details
    ISSN: 1617-4623
    Keywords: Protoplast culture ; Nuclear and cytoplasmic male sterility ; Mitochondrial DNA and proteins ; Nicotiana sylvestris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Male sterile plants appeared in the progeny of three fertile plants obtained after one cycle of protoplast culture from a fertile botanical line and two androgenetic lines ofNicotiana sylvestris. These plants showed the same foliar and floral abnormalities as the cytoplasmic male sterile (cms) mitochondrial variants obtained after two cycles of culture. We show that male sterility in these plants is controlled by three independent nuclear genes,ms1, ms2 andms3, while no changes can be seen in the mitochondrial genome. However, differences were found between thein organello mitochondrial protein synthesis patterns of male sterile and parent plants. Two reproducible changes were observed: the presence of a new 20 kDa polypeptide and the absence of a 40 kDa one. Such variations were described previously in mitochondrial protein synthesis patterns of the cms lines. Fertile hybrids of male sterile plants showed normal synthesis patterns. The male sterile plants are thus mutated in nuclear genes involved in changes observed in mitochondrial protein synthesis patterns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00633819
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Amplification of substoichiometric recombinant mitochondrial DNA sequences in a nuclear, male sterile mutant regenerated from protoplast culture inNicotiana sylvestris (1992)
    Springer
    Molecular genetics and genomics 233 (1992), S. 193-200 
    Details
    ISSN: 1617-4623
    Keywords: Protoplast culture ; Nuclear male sterility ; Mitochondrial DNA recombination ; Substoichiometric molecules ; Nicotiana sylvestris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary ANicotiana sylvestris plant regenerated from protoplast culture was found to be mutated in both the mitochondrial (mt) and nuclear genomes. The novel mt DNA organization, called U, is due to the amplification of recombinant substoichiometric DNA sequences that preexist in the parent line. The recombination event involves two 404 by repeats, which hybridize to a 2.1 kb transcript. Although the sequence of both repeats was not altered by the recombination, an additional transcript of 2.5 kb was detected in U mitochondria. In addition to this mitochondrial reorganization, the protoclone carried a recessive nuclear mutation conferring male sterility (ms4). A possible role ofms4 in the appearance of the U mt DNA organization was investigated by introducing this gene into normalN. sylvestris cytoplasm. No mt DNA change could be found in homozygousms4/ms4 plants of the F2 generation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587579
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    Paper (German National Licenses)
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