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  • 1
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    In:  Notes from the Leyden Museum (1872-9231) vol.6 (1884) nr.3 p.167
    Publication Date: 2014-11-24
    Description: During a visit to the Leyden Museum last September I was able to examine several species of birds, which had not come under my notice during the preparation of the 7th volume of the »Catalogue of Birds”. Some of the species proved to be very distinct and I greatly regret that they have been omitted from the volume or relegated to foot-notes, as being of doubtful validity. During the whole of the time that this volume was going through the press, I was engaged in the moving of the Zoological Collections from Bloomsbury to South Kensington, and was unable to get abroad for my accustomed course of study in foreign Museums. I am sensible that the value of this volume has been somewhat impaired by these omissions, and I publish these supplementary notes, in order to supply descriptions of several species which are wanting to its pages. Ale the diademata (Bp.), Sharpe, 1. c. p. 58. Turdus diadematus, Temm. MSS. in Mus. Lugd., undè Bessonornis diadematus, Bp. Consp. I, p. 302.
    Repository Name: National Museum of Natural History, Netherlands
    Type: Article / Letter to the editor
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  • 2
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    In:  Notes from the Leyden Museum vol. 6 no. 3, pp. 179-180
    Publication Date: 2024-01-12
    Description: In the Leyden Museum are some specimens of a bird from Timor, bearing the label \xc2\xab Orthotomus subulatus, M\xc3\xbcll. Verh.\xc2\xbb, a name which I cannot find to have been published anywhere, and as far as I am aware the species has never been described under any other name.\nGenerically this Wren comes very close to Pnoepyga, and has no rictal bristles and the front aspect of the tarsus entire. It has, however, a much longer bill, thinner, and more compressed, the culmen exceeding the hind toe and claw in length. I propose to separate it generically as Orthnocichla. The type will be
    Repository Name: National Museum of Natural History, Netherlands
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  • 3
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    In:  Notes from the Leyden Museum vol. 10 no. 3, pp. 199-200
    Publication Date: 2024-01-12
    Description: During my recent visit to the Leyden Museum I was much interested in examining the fine series of birds\xe2\x80\x99-skins which had been brought home by Mr. B\xc3\xbcttikofer and Mr. Stampfli from their expeditions to Liberia.\nAmongst other valuable specimens I was particularly attracted by a small Sparrow-Hawk, of which Mr. B\xc3\xbcttikofer had preserved three adults, two females and a male. These are identified by him as Accipiter hartlaubi of Verreaux \xc2\xb9), but I saw at once that they were not the same as the bird I had so named in the Catalogue of Birds (Vol. I, p. 150; pl. 6, fig. 2).
    Repository Name: National Museum of Natural History, Netherlands
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  • 4
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    In:  Notes from the Leyden Museum vol. 6 no. 3, pp. 167-178
    Publication Date: 2024-01-12
    Description: During a visit to the Leyden Museum last September I was able to examine several species of birds, which had not come under my notice during the preparation of the 7th volume of the \xc2\xbbCatalogue of Birds\xe2\x80\x9d. Some of the species proved to be very distinct and I greatly regret that they have been omitted from the volume or relegated to foot-notes, as being of doubtful validity. During the whole of the time that this volume was going through the press, I was engaged in the moving of the Zoological Collections from Bloomsbury to South Kensington, and was unable to get abroad for my accustomed course of study in foreign Museums. I am sensible that the value of this volume has been somewhat impaired by these omissions, and I publish these supplementary notes, in order to supply descriptions of several species which are wanting to its pages.\nAle the diademata (Bp.), Sharpe, 1. c. p. 58. Turdus diadematus, Temm. MSS. in Mus. Lugd., und\xc3\xa8 Bessonornis diadematus, Bp. Consp. I, p. 302.
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of ornithology 30 (1882), S. 344-347 
    ISSN: 1439-0361
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-0878
    Keywords: Testis ; Sertoli cells ; Testosterone ; Hypophysectomy ; Ethane dimethanesulphonate ; Rat (Sprague-Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The morphological response of the Sertoli cells to partial or complete withdrawal of testosterone was studied in adult rats following hypophysectomy or administration of ethane dimethanesulphonate (EDS), a toxicant known to destroy selectively the Leydig cells of the testis. To assess the role of germ cells in effecting changes to Sertoli cells following withdrawal of testosterone, germ cell-deficient rats with Sertoli-cell-only testes (SCO) were treated with EDS to remove the source of testosterone. At 6 days after hypophysectomy or 4,6 and 8 days after EDS treatment, stage VII and VIII seminiferous tubules showed degenerating germ cells and numerous basally-located vacuoles approximately 1–15 μm in diameter. Ultrastructural analysis indicated that most of the vacuoles were multiple focal dilations of the intercellular space associated with Sertoli cell junctional complexes. In SCO rats, treatment with EDS resulted in a significant (P〈0.05) increase in the formation of many vacuoles particularly in the base but also in the trunk of the Sertoli cells and again electron microscopic analysis showed multiple, localized expansions of the intercellular space associated with Sertoli cell junctional complexes. The appearance of intercellular spaces in SCO testes following androgen withdrawal cannot be attributed to shrinkage of degenerating germ cells since the seminiferous tubules did not contain germ cells. It is concluded that withdrawal of androgen induces early morphological alterations of the Sertoli cell junctional complexes in which the sites of membrane fusions representing tight junctions remain intact whereas the intercellular spaces exhibit major focal dilations. The results are discussed in relation to the fluid secretion by the seminiferous tubules which is regulated by the Sertoli cells.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-0878
    Keywords: Testis ; Spermatogenesis ; FSH ; Testosterone ; Rat (Sprague-Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adult rats were hypophysectomized and treated with ethane dimethanesulphonate (EDS) selectively to eliminate the Leydig cells in the testis. By removing the source of endogenous gonadotrophins and androgens, the subsequent effects on the seminiferous epithelium were studied after 20 days of treatment with vehicle, or FSH (2x50 μg/day) or a low dose of testosterone (0.6 mg testosterone esters every 3rd day) alone or in combination. Compared to vehicle-treated hypophysectomized rats with Leydig cells, testis weight in saline-treated hypophysectomized rats treated with EDS declined by 50%, spermatogenesis was disrupted severely and only 18% of the tubules contained spermatids, these being confined to stages I–VI of the spermatogenic cycle. Treatment with either FSH or testosterone esters alone significantly (P〈0.01) increased testis weight compared to vehicle-treated hypophysectomized rats treated with EDS and 40% of tubules contained spermatids either at stages I–VI after FSH, or at all stages I–XIV after testosterone treatment. Treatment with FSH and testosterone esters together maintained testis weights approximately 20% above vehicle-treated hypophysectomized controls; over 70% of the seminiferous tubules contained spermatids and there was a marked stimulation of spermatogenesis at all stages of the spermatogenic cycle. The results suggest, that in the absence of the pituitary gland and the Leydig cells, FSH alone partially supports spermatogenesis up to the development of round spermatids whereas testosterone is capable of maintaining spermatid development at all 14 stages of the cycle. When FSH and testosterone were administered in combination, the effects upon spermatogenesis were far greater than the response expected if their individual effects were simply additive. It is therefore concluded that FSH may play a role in normal spermatogenesis and that this role is essentially that of augmenting the response of the testis to testosterone. The biochemical mechanisms via which this might occur are discussed and hypophysectomized rats treated with EDS used in the present studies should provide a useful approach for their identification.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-0878
    Keywords: Spermatogenesis ; Cyclic protein 2 ; Transition protein 2 ; Cytochrome c oxidase ; Sertoli cell ; Spermatids ; Pachytene spermatocytes ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In an attempt to identify key changes involved in normal spermatogenesis we have developed methods to enable the study of gene expression by the various subpopulations of testicular cells by use of in-situ hybridisation histochemistry. The use of digoxigenin-labelled ribonucleotide and oligonucleotide probes on testicular tissue perfusion-fixed with Bouin's fixative and embedded in paraffin, polystyrene or methacrylate, has been used to accurately localise three transcripts to three different cell types (Sertoli cells, pachytene spermatocytes, and step 7–12 spermatids) within the seminiferous tubule. The ability to produce semi-thin sections of polystyrene- or methacrylate-embedded tissue and successfully to apply digoxigenin-labelled ribonucleotide or oligonucleotide probes resulted in far greater resolution and unequivocal localisation of mRNA in testicular cells than was previously possible by use of thicker paraffin or frozen sections hybridised with 35S-labelled riboprobes. A comparison of the different embedding media versus digoxigenin-labelled oligonucleotide or ribonucleotide probes is made and we demonstrate the relative sensitivities and merits of each combination.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 0003-276X
    Keywords: Spermatogenesis ; Testosterone ; Germ cell degeneration ; Testis ; Rat ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Rapid and complete withdrawal of intratesticular testosterone was achieved via the destruction of all Leydig cells with the specific Leydig cell cytotoxin ethane dimethanesulphonate (EDS). Restoration of testosterone levels was accomplished by administration of a single dose (25 mg) of testosterone esters (T) known to reverse the antispermatogenic effects of androgen withdrawal. Quantitation of the degenerating germ cells in cross sections of seminiferous tubules (ST) at stages IV-V, VII, IX, and X-XI of the spermatogenic cycle was used as a sensitive biological index of the effects of testosterone withdrawal and restoration upon the function of the Sertoli cells. Compared to control testicular tissues, the mean numbers of pyknotic germ cells per ST cross section at stages VII, IX and X-XI increased significantly (P 〈 0.01-0.001) between 4 to 8 days post-EDS treatment, but only in stage VII tubules was this trend reversed significantly (P 〈 0.005) within 2 days by T supplementation. In EDS-treated rats, stages VII, VIII, IX, and X-XI also exhibited significant (P 〈 0.05-0.001) increases (compared to controls) in the volumetric proportions by which intraepithelial vacuoles appeared within the seminiferous tubules. Again, in EDS+T supplemented rats, the appearance of vacuoles was significantly (P 〈 0.001) suppressed in stage VII and VIII. In contrast to tubules at stages VII-XI, those at stages IV-V were completely unaffected by testosterone withdrawal or replacement. The results show that at selected time intervals after EDS treatment, testosterone supplementation is capable of preventing/reversing these morphological changes within 2 days in stage VII tubules. It is suggested that the induction and subsequent prevention of seminiferous epithelial damage will serve as an important in vivo and in vitro approach for studies on the androgen-mediated changes in Sertoli cell biology during phases of impairment and recovery of their function. Manipulation of adult Sertoli cell function as provided by our model should permit identification of androgen-regulated gene products together with an understanding of their role(s) in normal and abnormal spermatogenesis. © 1993 Wiley-Liss, Inc.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1040-452X
    Keywords: Spermatogenesis ; Digoxigenin ; TP2 ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The present study has used methoxyacetic acid (MAA)-induced depletion of specific germ cell types in the rat and in situ hybridization with nonradioactive riboprobes to determine the stages of the spermatogenic cycle at which there is expression of the mRNA for the basic chromosomal protein transition protein 2 (TP2). On Northern blots, an abundant mRNA was detectable in samples from control adult rats, but the amount of message was markedly reduced when RNA was extracted from the testes of rats treated 14 and 21 days previously with methoxyacetic acid. These testes were depleted specifically of step 7-12 spermatids, suggesting that these cells contain TP2 mRNA. When tissue sections were subjected to in situ hybridization, the TP2 mRNA was localized at the cellular and subcellular levels. Messenger RNA for TP2 was first detectable in spermatids at step 7. In these spermatids, at high magnification, in addition to some positive reaction in the cytoplasm, intense staining was located to a perinuclear structure consistent with localization of mRNA within the chromatoid body. The amount of TP2 mRNA in the cytoplasm increased as remodelling of the early spermatid nucleus progressed and was highest in step 10 and 11 spermatids at stages X and XI. Thereafter, the mRNA decreased until it was undetectable in step 14 spermatids at stage XIV. The localization of TP2 mRNA to the chromatoid body of step 7 spermatids would be consistent with this organelle being a storage site for long-lived mRNAs utilized later in spermiogenesis. © 1992 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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