ISSN:
1573-5028
Keywords:
PEP carboxylase
;
housekeeping gene
;
gene expression
;
gene structure
;
light-mediated activation
;
Saccharum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A gene (SCPEPCD1) encoding phosphoenolpyruvate carboxylase (PEPC) was isolated from the C-4 monocot sugarcane (Saccharum hybrid var. H32-8560). SCPEPCD1 is ca. 6800 bp long, with 10 exons. The entire gene sequence from −1561 to 262 bp downstream of the putative poly(A) addition signal is reported. A low-level, essentially constitutive pattern of expression, amino acid sequence similarities to other ‘housekeeping’ PEPC enzymes, and the absence of DNA sequence elements conserved in the upstream region of maize and sorghum C-4-specific PEPC genes indicate that SCPEPCD1 encodes a housekeeping PEPC. Despite this, a motif proposed to act as a phosphorylation site in light-mediated activation of photosynthetic PEPC enzymes [10] is present in the SCPEPCD1 protein; evidence is presented for the presence of this site in other housekeeping PEPC proteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00046451
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