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  • 1
    Electronic Resource
    Electronic Resource
    Do genealogical patterns in purple photosynthetic bacteria reflect interspecific gene transfer? (1980)
    [s.l.] : Nature Publishing Group
    Nature 283 (1980), S. 212-214 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The conclusion of Ambler et al.1, suggesting that the relationships seen among cytochrome c sequences of the Rhodospirillaceae are merely due to random lateral transfer of the relevant gene, is based on three arguments. First, within the purple photosynthetic bacteria there is 'no correlation ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/283212a0
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  • 2
    Electronic Resource
    Electronic Resource
    Ammonia translocation in cyanobacteria (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 88 (1991), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Ammonia is the inorganic nitrogen source preferentially used by most cyanobacteria. Moreover, even in nitrate utilizers or N2-fixers, ammonia is an obligate intracellular intermediate in nitrogen assimilation. It also affects the synthesis and the activation of several key enzymes in nitrogen metabolism such as nitrogenase, glutamine synthetase and nitrate reductase. The mechanism by which NH3/NH4+ enters different cyanobacteria (N2-fixers, non-fixers, neutrophilic) was thus studied. Using 14CH3NH3+, the convenient radioactive analog of ammonium, we have shown that the neutrophilic A. nidulans R-2 possibly possesses an active transport system for this cation. The conditions leading to repression and depression of this transport system have been studied; it appears that de novo protein synthesis is required for the acquisition of the transport ability. We have also provided evidence that methionine sulfoximine affects ammonium uptake only through its inhibition on glutamine synthetase, and found no support for the possible interaction of this inhibitor with the ammonium transporter. In the alkalophilic cyanobacterium Spirulina platensis, an active mechanism to translocate ammonium is probably not needed. Our data suggest that net uptake of ammonia to support optimal growth could be explained by a pH driven diffusion process.In all the different strains tested net ammonia uptake was observed only when conditions permit continuous amidation through the activity glutamine synthetase and inhibition of this enzyme by methionine sulfoximine caused the excretion of ammonia to the external medium. The weight of experimentation suggests that ammonia leaks from cells because of the inherent permeability of NH3, and that no specific carrier is involved in its release.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04953.x
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of light on growing and starved populations of extremely halophilic bacteria (1982)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 14 (1982), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1982.tb08654.x
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  • 4
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    Dianne Newell and Ralph Greenhill, "Survivals: Aspects of Industrial Archaeology in Ontario" (Book Review) (1991)
    Detroit, Mich. : Periodicals Archive Online (PAO)
    Technology and Culture. 32:2 (1991:Apr.) 414 
    Details
    ISSN: 0040-165X
    Topics: Technology
    Notes: BOOK REVIEWS
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:5129-1991-032-02-000020
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  • 5
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    Peter Mathias and John A. Davis, eds., "The First Industrial Revolutions" (Book Review) (1993)
    Detroit, Mich. : Periodicals Archive Online (PAO)
    Technology and Culture. 34:3 (1993:July) 682 
    Details
    ISSN: 0040-165X
    Topics: Technology
    Notes: BOOK REVIEWS
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:5129-1993-034-03-000022
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  • 6
    Electronic Resource
    Electronic Resource
    Movement of acetate across the cytoplasmic membrane of the unicellular cyanobacteria Synechococcus and Aphanocapsa (1981)
    Springer
    Archives of microbiology 130 (1981), S. 175-179 
    Details
    ISSN: 1432-072X
    Keywords: Acetate ; pH gradients ; Membrane permeability ; Cyanobacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mutants of Synechococcus and of Aphanocapsa which were unable to activate acetate have been used to demonstrate that acetate entered the cells rapidly in darkness, and to a greater extent in light. Total internal concentrations under different conditions can be explained if acetic acid equilibrates rapidly across the cell membrane while acetate ion is strongly hindered. Acetate as well as other weak acids such as 5,5-dimethyl-2,4-oxazolidenedione can therefore be used as a probe of internal pH in these mutants. The intracellular pH was maintained at about 7.1 in darkness and 7.6 in light when external pH was varied from 6.8–8.0 No carrier involved in acetic acid equilibration could be demonstrated. Of other organic acids investigated, only propionate distributed in accordance with pH differences between the cells and surrounding fluid. The low uptake rates of glycolate, pyruvate and leucine appeared limited by slow movement of molecules into the cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411073
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  • 7
    Electronic Resource
    Electronic Resource
    Chloroherpeton thalassium gen. nov. et spec. nov., a non-filamentous, flexing and gliding green sulfur bacterium (1984)
    Springer
    Archives of microbiology 138 (1984), S. 96-101 
    Details
    ISSN: 1432-072X
    Keywords: Green sulfur bacterium ; Flexing ; Gliding ; Obligate phototroph ; Bacteriochlorophyll c
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A flexing and gliding green sulfur bacterium has been isolated from marine sources off the North East coast of the USA. Chloroherpeton thalassium is an obligate phototroph, and requires CO2 and S2- for growth; some organic acids can contribute to cell carbon, and N2 may be fixed. The cells contain typical chlorosomes, and gas vesicles may be present. Bacteriochlorophyll c is the main light harvesting pigment, and a small quantity of bacteriochlorophyll a is also present. Over 80% of the carotenoid is γ-carotene. DNA base composition of the isolates ranges from 45.0–48.2 mol% G+C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413007
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  • 8
    Electronic Resource
    Electronic Resource
    Filamentous growth of Escherichia coli K12 elicited by dimeric, mixed-valence complexes of ruthenium (1984)
    Springer
    Archives of microbiology 139 (1984), S. 265-271 
    Details
    ISSN: 1432-072X
    Keywords: Cell division ; Escherichia coli ; Ruthenium compounds ; Filament formation ; Mutagenesis ; Cell cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dimeric, mixed-valence [(Ru(II), Ru(III)] compounds of ruthenium caused filament formation in growing cultures of Escherichia coli K12. Three compounds with the general formula Ru2(NH3)6X5 · H2O (where X is a halide) were tested; in order of decreasing effectiveness (and with the concentration giving maximum effect), these were the bromo (10-5 M), chloro (10-4 to 10-5 M), and iodo (10-3 to 10-4 M) analogues. Filamentation elicited by the bromo and chloro compounds was spontaneously reversible after 3–4 h, and tentatively attributed to oxidation of the active mixed-valence form to inactive Ru(III) complexes. Several compounds known to accelerate division of filaments formed under other conditions were ineffective in reversing the filamentation, but the presence of 0,43 M-dimethylsulphoxide totally inhibited filamentation caused by the bromo or chloro compounds and by cis-Pt(NH3)2Cl2 (cisplatin), an established filamenting and antitumour agent. The ruthenium complexes bound to mammalian DNA, but were without effect on the UV spectrum or cellular content of DNA in E. coli, despite showing marked mutagenic activity in reverse mutation tests with Salmonella typhimurium. Cells remained sensitive to inhibition of division by the ruthenium complexes until immediately prior to the division event. Possibilities for the (probably complex) mode of action and the potential of related compounds for therapeutic use are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402012
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  • 9
    Electronic Resource
    Electronic Resource
    Endogenous ammonia production by Anacystis nidulans R-2 induced by methionine sulfoximine (1984)
    Springer
    Archives of microbiology 138 (1984), S. 217-219 
    Details
    ISSN: 1432-072X
    Keywords: Ammonia production ; Protein breakdown ; Anacystis nidulans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Anacystis nidulans R-2 produced ammonia from endogenous sources for at least 6 h when illuminated without external nitrogen source but with CO2 in the presence of 50 μM methionine sulfoximine. The onset of ammonia release coinciding with complete inhibition of glutamine synthetase. The total quantity of ammonia which could be released exceeded the nitrogen content of small molecule pools, and suggested protein degradation as the most likely source of the nitrogen. Ammonia release was not accompanied by leakage of carbon compounds from the cells. Methionine sulfoximine-induced ammonia release was energy requiring, and was barely detectable under dark anaerobic conditions, or in the presence of 10 μM carbonyl cyanide m-chlorophenylhydrazone in light. Phenyl methyl sulfonylfluoride, an inhibitor of serine proteases, eliminated ammonia release, and the rate of release was reduced to one-third of control values, after a lag, in the presence of 50–75 μg/ml chloramphenicol. The rate of NH + 4 release was maximal (1.4 nmol·min-1·mg-1 protein) if suspensions were bubbled with 100% O2, but could not be reduced below 0.6 nmol·min-1·mg-1 protein in air: CO2, suggesting that release was at most only partly due to photorespiration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402123
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  • 10
    Electronic Resource
    Electronic Resource
    Chromatin structure changes suggest a compensatory response to c-myc gene amplification in malignant fibrous histiocytoma (1992)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 49 (1992), S. 148-156 
    Details
    ISSN: 0730-2312
    Keywords: c-myc transcription ; DNAse I hypersensitivity ; oncogenes ; sarcomas ; tumor cell lines ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Changes in chromatin structure as determined from DNAse I hypersensitive site analysis are associated with c-myc amplification and increased transcript/protein levels in malignant fibrous histiocytoma (MFH) cell lines. A DNAse I hypersensitive site near the PO promoter region was observed in one MFH cell line (UR HCL 1), and in normal fibroblasts (HFF), but not in an MFH cell line with an amplified c-myc gene (P3C). A DNAse I hypersensitive site exclusive to P3C amplified c-myc was identified slightly 3′ of exon one. No alterations in c-myc DNAse I hypersensitive site patterns were observed in HFF fibroblasts following serum release, when peak levels of c-myc transcript were induced. DNAse I hypersensitive site patterns associated with gene amplification may reflect a compensatory response by P3C cells to an abundance of c-myc transcript. Furthermore, elevated levels of protein in P3C cells provide additional evidence that amplified c-myc is an oncogene in MFHs.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240490207
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