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  • 1995-1999  (3)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    A confocal fiber-coupled single-lens theta microscope (1998)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 69 (1998), S. 2956-2963 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A confocal theta microscope using a single water-immersion objective lens is described. The system is based on the Zeiss Axioplan universal microscope, such that the illumination light is coupled into, and the detected light out of, the microscope optics via optical fibers attached to the reflector slider of the microscope. Conventional wide-field, laser-scanning confocal, confocal theta, and 4Pi-confocal theta microscopy modes are available with the system. As the design can be easily adapted to other microscopes, objective lenses, and wavelengths, it allows confocal theta techniques to be implemented in many standard systems. The design constraints and specifications for the microscope are given, as well as a demonstration of its performance. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1149040
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    High-resolution axial and lateral position sensing using two-photon excitation of fluorophores by a continuous-wave Nd:YAG laser (1996)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 69 (1996), S. 446-448 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The change in position of fluorescent beads captured inside the focal volume of optical tweezers is monitored using fluorescence emission induced by two-photon absorption of a continuous-wave Nd:YAG laser (λ=1064 nm). The displacement of a bead due to interactions with its environment leads to a fluorescence intensity variation that is used to design a novel spatial sensor. We determine changes in the axial position of a CY3-labeled latex bead with a diameter of 1.03 μm to a precision better than 10 nm. At an intensity of 600 mW/ μm2 the two-photon bleaching rate is lower than 50% per 2000 s. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.118134
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  • 3
    Electronic Resource
    Electronic Resource
    Structure and dynamics of human interphase chromosome territories in vivo (1998)
    Springer
    Human genetics 〈Berlin〉 102 (1998), S. 241-251 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A new approach is presented which allows the in vivo visualization of individual chromosome territories in the nuclei of living human cells. The fluorescent thymidine analog Cy3-AP3-dUTP was microinjected into the nuclei of cultured human cells, such as human diploid fibroblasts, HeLa cells and neuroblastoma cells. The fluorescent analog was incorporated during S-phase into the replicating genomic DNA. Labelled cells were further cultivated for several cell cycles in normal medium. This well-known scheme yielded sister chromatid labelling. Random segregation of labelled and unlabelled chromatids into daughter nuclei resulted in nuclei exhibiting individual in vivo detectable chromatid territories. The territories were composed of subcompartments with diameters ranging between approximately 400 and 800 nm which we refer to as subchromosomal foci. Time-resolved in vivo studies demonstrated changes of positioning and shape of territories and subchromosomal foci. The hypothesis that subchromosomal foci persist as functionally distinct entities was supported by double labelling of chromatin with CldU and IdU, respectively, at early and late S-phase and subsequent cultivation of corresponding cells for 5–10 cell cycles before fixation and immunocytochemical detection. This scheme yielded segregated chromatid territories with distinctly separated subchromosomal foci composed of either early- or late-replicating chromatin. The size range of subchromosomal foci was similar after shorter (2 h) and longer (16 h) labelling periods and was observed in nuclei of both living and fixed cells, suggesting their structural identity. A possible functional relevance of chromosome territory compartmentalization into subchromosomal foci is discussed in the context of present models of interphase chromosome and nuclear architecture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050686
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