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  • 1
    Electronic Resource
    Electronic Resource
    Identification of Tn10 insertions in the dsbA gene affecting Escherichia coli biofilm formation (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 173 (1999), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Escherichia coli was used as model to study initial adhesion and early biofilm development to an abiotic surface. Tn10 insertion mutants with reduced attachment to a polystyrene surface were isolated. Three adhesion mutants harbored the transposon in the dsbA gene, whose product, DsbA, catalyses folding of numerous extracytoplasmic disulfide bond-containing proteins. All three mutants were weakly adherent and grew poorly. Cell surface structure analysis showed that motility, type 1 fimbriation and lipopolysaccharide structure were affected in these mutants. The pleiotropic effect of the dsbA mutations on biofilm formation is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1999.tb13532.x
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of Tn10 insertions in the rfaG, rfaP, and galU genes involved in lipopolysaccharide core biosynthesis that affect Escherichia coli adhesion (1999)
    Springer
    Archives of microbiology 172 (1999), S. 1-8 
    Details
    ISSN: 1432-072X
    Keywords: Key words Bacterial adhesion ; Biofilm formation ; Abiotic surface ; Type 1 fimbriae ; Flagella ; Lipopolysaccharide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Escherichia coli was used as a model to study initial adhesion and early biofilm development to abiotic surface. Tn10 insertion mutants of Escherichia coli K-12 W3110 were selected for altered abilities to adhere to a polystyrene surface. Seven insertion mutants that showed a decrease in adhesion harbored insertions in genes involved in lipopolysaccharide (LPS) core biosynthesis. Two insertions were located in the rfaG gene, two in the rfaP gene, and three in the galU gene. These adhesion mutants were found to exhibit a deep-rough phenotype and to be reduced, at different levels, in type 1 fimbriae production and motility. The loss of adhesion exhibited by these mutants was associated with either the affected type 1 fimbriae production and/or the dysfunctional motility. Apart from the pleiotropic effect of the mutations affecting LPS on type 1 fimbriae and flagella biosynthesis, no evidence for an involvement of the LPS itself in adhesion to polystyrene surface could be observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050732
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  • 3
    Electronic Resource
    Electronic Resource
    Use of a luminescent bacterial biosensor for biomonitoring and characterization of arsenic toxicity of chromated copper arsenate (CCA) (1997)
    Springer
    Biodegradation 8 (1997), S. 105-111 
    Details
    ISSN: 1572-9729
    Keywords: Escherichia coli ; ars operon ; biosensor ; CCA ; wood preservative ; arsenic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An arsenic oxyanion-inducible Escherichia colichromosomal operon (arsRBC) has been previouslyidentified. Construction of a luciferasetranscriptional gene fusion (arsB::luxAB) showedthat ars operon expression, plus concomitantcell luminescence, was inducible in a concentration-dependent manner by arsenic salts. The present studywas conducted to evaluate the potential of the arsB::luxAB transcriptional gene fusion for use as abiosensor in monitoring the toxicity of arseniccompounds. Cultures from this gene fusion strain wereexposed to increasing concentrations of the woodpreservative chromated copper arsenate (CCA), as wellas its constituents, sodium arsenate and chromatedcopper solution (CC). Analysis of luciferase activityrevealed that the arsB::luxAB gene fusion wasexpressed in response to CCA and sodium arsenate, butnot to the CC solution. The detection limit of arsenicwas found to be 0.01 µg As/ml (10 parts perbillion, 10 ppb) and therefore well within the rangeof environmental concerns. A greater induction ofluminescence by arsenate was observed when cells werelimited for phosphate, as phosphate can act as acompetitive inhibitor of arsenate ions. Our resultssuggest that the E. coli arsB::luxAB fusionstrain has a promising future as a specific andsensitive biosensor for monitoring bioavailable levelsand toxicity of arsenic near sites where CCA-treatedwood has been used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008281028594
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