In:
Genome, Canadian Science Publishing, Vol. 42, No. 1 ( 1999-02-01), p. 48-51
Abstract:
Digoxigenin-labeled total genomic DNA from Oryza eichingeri was hybridized in situ to somatic chromosome preparations of F 1 , F 2 , backcross progenies, and a pollen-derived tetraploid plant (E 24 ) from O. sativa (2n = 24, genome AA) × O. eichingeri (2n = 24, genome CC), which allowed a definitive discrimination between A- and C-genome chromosomes. Twelve chromosomes in F 1 , F 2 , BC 1 , and twenty-four chromosomes in plant E 24 were clearly revealed to be of O. eichingeri origin, thus confirming that both BC 1 and F 2 were allotriploids (2n = 36, AAC) while plant E 24 was an amphiploid (2n = 48, AACC). In addition, the presence of O. eichingeri chromosomes in four alien addition lines was characterized. The results suggest that FISH/GISH may be a powerful tool for monitoring the O. sativa-alien chromosome in the progenies of rice interspecific hybridization.Key words: fluorescent in situ hybridization, Oryza sativa, Oryza eichingeri, amphiploid, alien addition lines.
Type of Medium:
Online Resource
ISSN:
0831-2796
,
1480-3321
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
1999
detail.hit.zdb_id:
2020635-5
SSG:
12
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