In:
Journal of Histochemistry & Cytochemistry, SAGE Publications, Vol. 45, No. 4 ( 1997-04), p. 481-491
Abstract:
Biotin was recently applied to detect cellular DNA or RNA. In combination with avidin, streptavidin or antibody, it can be conjugated with fluorescent dye, enzyme, ferritin, or gold. However, emphasis has recently been placed on the false-positive results that are obtained when this probe is used, because endogenous biotin may sometimes interfere with specific signals. Digoxigenin appears to be an interesting alternative because it is present exclusively in Digitalis plants as a secondary metabolite. We discuss in this review the efficiency and the respective advantages and disavantages of these two probes for in situ hybridization, mainly at the electron microscopic level.
Type of Medium:
Online Resource
ISSN:
0022-1554
,
1551-5044
DOI:
10.1177/002215549704500401
Language:
English
Publisher:
SAGE Publications
Publication Date:
1997
detail.hit.zdb_id:
1421306-0
SSG:
12
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