In:
Review of Scientific Instruments, AIP Publishing, Vol. 66, No. 6 ( 1995-06-01), p. 3520-3526
Abstract:
An apparatus for variable-angle ultraviolet total internal reflection fluorescence (TIRF) spectroscopy is described. TIRF spectroscopy is an analytical technique based on the excitation of fluorescence from a sample by means of an exponentially decaying surface energy wave. The limited penetration depth of the excitation volume results in excitation of fluorescence only near the sample/substrate interface. The angular spectrum makes TIRF spectroscopy suitable for obtaining quantitative information on the depth profile of a sample. In the instrument described here, precise positioning of incident optics with correction of the chromatic aberration due to the xenon light source enables the measurement of the excitation spectrum. The system provides the fluorescence and excitation spectra for study of the physical and chemical properties of thin-film structures. The incident- and observation-angle dependences of those spectra give important information on the depth profiles of the fluorophores in the samples. The performance of the instrument is demonstrated with thin polymer films containing a fluorescent probe of pyrene. Pyrene monomer is found to form the nonuniform depth profile different from that of pyrene excimer.
Type of Medium:
Online Resource
ISSN:
0034-6748
,
1089-7623
Language:
English
Publisher:
AIP Publishing
Publication Date:
1995
detail.hit.zdb_id:
209865-9
detail.hit.zdb_id:
1472905-2
SSG:
11
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