In:
Journal of Leukocyte Biology, Oxford University Press (OUP), Vol. 64, No. 5 ( 1998-11-01), p. 631-635
Abstract:
Large and small macromolecular activators of phagocytosis from platelets (l-MAPP and s-MAPP, respectively), which function via the neutrophilic Fcy receptors (FcγR) were refined from platelet release products by gel filtration and affinity chromatography with the use of an anti transferrin antibody column and the mechanism of phagocytosis activation was investigated. Flow cytometry revealed that l-MAPP and s-MAPP did not increase the expression of neutrophilic FcγRII (CD32) and FcγRIII (CD16) antigens, whereas rosette formation of neutrophils with rabbit IgG-sensitized sheep erythrocytes (EA) in the presence of anti FcγR antibodies suggested that both MAPPs increase the binding ability of FcγRII. On the other hand, the enhancing effect of l-MAPP and s-MAPP on neutrophilic phagocytosis disappeared with the increase in phagocytosis by the phosphate-buffered saline control neutrophils when they were centrifuged with EA before incubation for phagocytosis. The enhanced phagocytosis, both by the two MAPPs and centrifugation, was canceled by treatment of the neutrophils with anti-CD32 Fab. The phagocytosis activatory effects of MAPP on neutrophils were canceled by anti-CD71 monoclonal antibody but not by transferrin. J. Leukoc. Biol. 64: 631–635; 1998.
Type of Medium:
Online Resource
ISSN:
0741-5400
,
1938-3673
DOI:
10.1002/jlb.64.5.631
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1998
detail.hit.zdb_id:
2026833-6
SSG:
12
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