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  • 1
    In: Plant Physiology, Oxford University Press (OUP), Vol. 121, No. 3 ( 1999-11-01), p. 913-919
    Abstract: A pyridoxal 5′-phosphate (PLP)-dependent enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (S-adenosyl-l-Met methylthioadenosine-lyase, EC 4.4.1.14), catalyzes the conversion ofS-adenosyl-l-methionine (AdoMet) to ACC. A tomato ACC synthase isozyme (LE-ACS2) with a deletion of 46 amino acids at the C terminus was chosen as the control enzyme for the study of the function of R286 in ACC synthase. R286 of the tomato ACC synthase was mutated to a leucine via site-directed mutagenesis. The ACC synthase mutant R286L was purified using a simplified two-step purification protocol. Circular dichroism (CD) analysis indicated that the overall three-dimensional structure of the mutant was indistinguishable from that of the control enzyme. Fluorescence spectroscopy revealed that the binding affinity of R286L ACC synthase for its cofactor PLP was reduced 20- to 25-fold compared with control. Kinetic analysis of R286L showed that this mutant ACC synthase had a significantly reduced turnover number (k  cat) of 8.2 × 10−3 s−1 and an increasedK  m of 730 μm for AdoMet, leading to an 8,000-fold decrease in overall catalytic efficiency compared with the control enzyme. Thus, R286 of tomato ACC synthase is involved in binding both PLP and AdoMet.
    Type of Medium: Online Resource
    ISSN: 1532-2548 , 0032-0889
    RVK:
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 1999
    detail.hit.zdb_id: 2004346-6
    detail.hit.zdb_id: 208914-2
    SSG: 12
    Location Call Number Limitation Availability
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  • 2
    Online Resource
    Online Resource
    Wiley ; 1999
    In:  Soil Science Society of America Journal Vol. 63, No. 5 ( 1999-09), p. 1174-1180
    In: Soil Science Society of America Journal, Wiley, Vol. 63, No. 5 ( 1999-09), p. 1174-1180
    Abstract: Information about soil P fractions is useful to predict the bioavailability of P in soil as well as to predict the likelihood of its transport. In this study, we used a sequential fractionation procedure to investigate the forms of P in a Mollisol amended at the soil surface with biosolids (i.e., anaerobically digested sewage sludge). Soil samples from three depths (0–5, 5–20, and 20–35 cm) were collected from a Cumulic Vertic Endoaquoll in a field experiment with three biosolid application rates, two vegetation treatments [hybrid poplar–cottonwood trees ( Populus × euramericana — clone NC‐5326) and switchgrass ( Panicum virgatum L.)], and four replications per treatment. The Hedley fractionation scheme (dividing soil P into six empirical fractions [water‐soluble, NaHCO 3 ‐soluble inorganic and organic P; NaOH‐soluble inorganic and organic P; HCl‐soluble P, and residual P)] was employed. After 6 yr of continuous application of biosolids to poplar plots, the absolute concentrations of all P fractions at the 0‐ to 5‐cm depth increased significantly ( P 〈 0.05). Some P fractions at the 5‐ to 20‐cm depth increased significantly, whereas at the 20‐ to 35‐cm depth, none of the fractions was affected by biosolids amendment. At the 0‐ to 5‐cm depth of both poplar tree and switchgrass plots, the relative concentrations of some of the P fractions (e.g., HCl–P, NaOH–OP, and residual P) decreased rather than increased. Because NaHCO 3 –IP and H 2 O–P increased in the biosolids‐amended soil at rates disproportionate to their concentrations in the biosolids, we conclude that HCl–P applied with biosolids was transformed to more labile forms.
    Type of Medium: Online Resource
    ISSN: 0361-5995 , 1435-0661
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 1999
    detail.hit.zdb_id: 196788-5
    detail.hit.zdb_id: 1481691-X
    SSG: 13
    SSG: 21
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  • 3
    In: Acta Physica Sinica, Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences, Vol. 44, No. 7 ( 1995), p. 1113-
    Type of Medium: Online Resource
    ISSN: 1000-3290 , 1000-3290
    Language: Unknown
    Publisher: Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences
    Publication Date: 1995
    detail.hit.zdb_id: 203490-6
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