In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 93, No. 26 ( 1996-12-24), p. 15260-15265
Abstract:
We have found conditions for saturation mutagenesis by restriction
enzyme mediated integration that result in plasmid tagging of disrupted genes. Using this method we selected for mutations in genes that act at
checkpoints downstream of the intercellular signaling system that controls encapsulation in Dictyostelium
discoideum . One of these genes, mkcA , is a
member of the mitogen-activating protein kinase cascade family while the other, regA , is a novel bipartite gene homologous to
response regulators in one part and to cyclic nucleotide phosphodiesterases in the other part. Disruption of either of these
genes results in partial suppression of the block to spore formation resulting from the loss of the prestalk genes, tagB and tagC . The products of the tag genes have
conserved domains of serine proteases attached to ATP-driven transporters, suggesting that they process and export peptide signals.
Together, these genes outline an intercellular communication system that coordinates organismal shape with cellular differentiation during
development.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.93.26.15260
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
1996
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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