In:
Protein & Peptide Letters, Bentham Science Publishers Ltd., Vol. 6, No. 6 ( 1999-12), p. 367-372
Abstract:
EPO Mimetic Peptide (EMP) encoding sequence was inserted into the gene of PAI-I between P2'-P3', generating a novel gene of PAI-1/EMP (PMP). It was cloned into pETlld expression vector, and a 43kDa protein was expressed in inclusion bodies with an expression level over 30%. The PMP protein was purified by DEAE ion-exchange chromatography to one band in SDS-PAGE (Silver Stained). The EPO activity of PMP was determined by Reticulocyte Counting Assay to be S000IU/mg, 2500 folds that of EMP (as reported).
Type of Medium:
Online Resource
ISSN:
0929-8665
DOI:
10.2174/092986650606221117124345
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
1999
Permalink