In:
European Journal of Endocrinology, Oxford University Press (OUP), Vol. 134, No. 2 ( 1996-02), p. 177-183
Abstract:
Salvatore D, Celetti A, Fabien N, Paulin C, Martelli ML, Battaglia C, Califano D, Monaco C, Viglietto G, Santoro M, Fusco A. Low frequency of p 53 mutations in human thyroid tumors; p 53 and Ras mutation in two out of fifty-six thyroid tumours. Eur J Endocrinol 1996;134:177–83. ISSN 0804–4643 Objective : p 53 is a well-known nuclear phosphoprotein encoded by a suppressor gene known to be mutated in various kinds of human tumours. A relationship between p 53 gene mutation and tumour progression seems to be a common feature of several neoplasias. Desing : In order to investigate the role of p 53 mutations in human thyroid tumours, DNA samples derived from fifty-six neoplastic tissues, ranging from benign adenomas to undifferentiated carcinomas, were examined for the presence of p 53 gene mutations. Methods : The analysis has been conducted using polymerase chain reaction (PCR) amplification of the exons 5–9 of the p 53 gene followed by single strand conformation polymorphism (SSCP) and sequence analyses. Results : One anaplastic carcinoma and one papillary carcinoma showed p 53 gene mutations in exons 5 and 8, respectively. A cell line established from the papillary carcinoma showed the same mutation present in the original tumour. Both p 53 mutations were heterozygous. The p 53 positive samples were analysed for other genetic alterations frequently detected in human thyroid carcinomas (mutations of the RET, TRK, and ras oncogenes): both p53-mutated samples proved to be mutated at level of codon 13 of the c-Ki- ras gene. Conclusions : Our data confirm that p 53 gene alterations are rare in well-differentiated thyroid tumours, that they are an important requirement for the establishment in culture of human thyroid carcinoma cell lines, and that they can be associated with other genetic alterations, namely ras mutations, in the malignant progression of thyroid tumours. Alfredo Fusco, Dipartimento di Biologia e Patologia Cellulare e Molecolare, Facoltà di Medicina e Chirurgia, via S. Pansini 5, 80131, Napoli, Italy
Type of Medium:
Online Resource
ISSN:
0804-4643
,
1479-683X
DOI:
10.1530/eje.0.1340177
Language:
Unknown
Publisher:
Oxford University Press (OUP)
Publication Date:
1996
detail.hit.zdb_id:
1485160-X
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