In:
Immunology & Cell Biology, Wiley, Vol. 73, No. 6 ( 1995-12), p. 575-583
Abstract:
To form distant metastases, tumour cells must stabilize adhesive interactions that prevent detachment at secondary sites. Primary receptor‐ligand interactions alone may not maintain prolonged adhesive contacts without secondary events that lead to adhesion stabilization. Computerized imaging methods enable us to examine various substrates for: (i) the wall shear adhesion threshold (WSAT). a measure of the dynamic adhesive potential of tumour cells; (ii) the number of tumour cells that adhered; and (iii) the adhesion stabilization lag time (ASLT) or length of time required for tumour cells to stabilize adhesive contacts capable of withstanding high wall shear force (up to 100 dynes/cm‐). The relative WSAT ratios found were: wheat germ agglutinin (WGA) 〉 laminin 〉 fibronectin 〉 vitronectin 〉 collagen I 〉 collagen IV 〉 von Willebrand factor (vWF) (the greater the shear rate the higher the adhesive potential). The relative stabilization ratios found were as follows: laminin 〈 fibronectin 〈 vitronectin 〈 collagen IV 〈 collagen I 〈 vWF 〈 WGA (shorter times correlate with greater stabilization potential). Stabilization data using fibronectin as a substrate correlated the best with metastatic potential. Using three melanoma lines of ditterent metastatic potential semi‐quantitative reverse transcriptase‐polymerase chain reaction (PCR) showed a two‐ to four‐fold increase in α1, α3, α4, α5, α6, and ICAM‐1 in the highly metastatic 70W cells compared to the Me Wo and non‐metastatic 3SS melanoma cells. There were no differences in α, β1 and β3 levels among the three melanoma lines, and PCR products for αIIb, α2, CD36, or ICAM‐2 were not detected. The 70W cells also had higher levels of αx and β2 (CD11/CD18 and pl50 leukocyte antigen) than either the MeWo or 3S5 cells. The data indicate that melanoma cells exhibit differences in the adhesion properties under fluid shear and differences in the expression of adhesion components that correlate with their metastatic potential.
Type of Medium:
Online Resource
ISSN:
0818-9641
,
1440-1711
Language:
English
Publisher:
Wiley
Publication Date:
1995
detail.hit.zdb_id:
2011707-3
SSG:
12
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