In:
Journal of Leukocyte Biology, Oxford University Press (OUP), Vol. 66, No. 4 ( 1999-10-01), p. 659-666
Abstract:
Interleukin-15 (IL-15) is a pleiotropic cytokine that induces the generation and differentiation of lymphoid cells and shares many biological activities with IL-2. We have shown here the development of dendritic cells (DC) from human CD34+ hemopoietic precursor cells cultured for 2–4 weeks with IL-15 alone. DC generated with IL-15 have typical morphological, immunocytochemical, phenotypic, and functional characteristics of mature DC. Dual flow cytometry analysis performed weekly demonstrated increasing co-expression of CD1a or CD83 with HLA-DR, CD80, CD86, IL-2R α, β, and γ. Two populations of cells were distinguished among CD34+ progeny. Small and medium-size cells were mainly natural killer (NK) cells (72.6–85.2% CD56+) and low numbers of DC (9.1–21.3% CD1a+). Large cells were mostly DC (75.4–95.4% CD1a+). Isolated CD34+ cells did not express IL-2R subunits but after 2–3 days in culture with IL-15, they were found to express IL-2Rγ. Induced expression of IL-2Rγ on CD34+ cells may explain the primary mechanism of IL-15-regulated differentiation of hemopoietic precursor cells. Thus, our data suggest that IL-15 stimulates CD34+ cells to differentiate into NK and DC and may represent a new growth and survival factor for lymphoid DC. J. Leukoc. Biol. 66: 659–666; 1999.
Type of Medium:
Online Resource
ISSN:
0741-5400
,
1938-3673
DOI:
10.1002/jlb.66.4.659
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1999
detail.hit.zdb_id:
2026833-6
SSG:
12
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