In:
International Journal of Urology, Wiley, Vol. 5, No. 6 ( 1998-11), p. 595-600
Abstract:
Background: In addition to its role in calcium and phosphorus metabolism, calcitriol (1,25‐dihydroxyvitamin D; 1,25‐D3) demonstrates multiple effects on cell proliferation/differentiation by expressing major histocompatibility complex (MHC) antigens and intercellular adhesion molecule‐1 (ICAM‐1). It has recently been reported that 1,25‐D3 inhibits the growth of prostatic cancer (PCa) cells. In this study we examined the effect of 1,25‐D3 on both the growth and expression of HLA‐ABC, HLA‐DR and ICAM‐1 antigens in PCa cells. Methods: Four human PCa cell lines (PC‐3, PPC‐1, ALVA‐41 and ALVA‐101) were examined. The cell numbers were enumerated, and the effects of interferon‐y (IFN‐y) and 1,25‐D3 on the expression of HLA‐ABC, HLA‐DR and ICAM‐1 were quantitated by flow cytometry. Results: A dose‐dependent antiproliferative effect of 1,25‐D3 was found in all PCa eel Is lines except ALVA‐41. 1,25‐D3 was approximately 10 times as potent as its analogue 24,25‐dihydroxyvitam in D 3 in inhibiting the growth of PC‐3 cells. Also, the relative inhibitory ability of these compounds paralleled the strength of their binding affinities for the 1,25‐D3 receptor, indicating that the antiproliferative effect may require a receptor‐ligand interaction. HLA‐ABC was expressed in PC‐3, ALVA‐41 and ALVA‐101, but not in PPC‐1 cells, while HLA‐DR was not expressed on any of the tested cells. IFN‐y could enhance or induce HLA‐ABC but not HLA‐DR expression in the tested cells. ICAM‐1 was expressed in all cells and slightly up‐regulated by IFN‐y. Conclusion: In this study 1,25‐D3 had an antiproliferative effect on 3 of the 4 examined PCa cell lines.
Type of Medium:
Online Resource
ISSN:
0919-8172
,
1442-2042
DOI:
10.1111/iju.1998.5.issue-6
DOI:
10.1111/j.1442-2042.1998.tb00418.x
Language:
English
Publisher:
Wiley
Publication Date:
1998
detail.hit.zdb_id:
2009793-1
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