In:
Journal of Parenteral and Enteral Nutrition, Wiley, Vol. 22, No. 1 ( 1998-01), p. 31-36
Abstract:
Background: IV administration of a total parenteral nutrition (TPN) solution results in small intestinal gut‐associated lymphoid tissue (GALT) atrophy, lowers small intestinal immunoglobulin A (IgA) levels, and impairs upper respiratory tract secretory IgA‐mediated mucosal immunity; isonitrogenous supplementation of TPN with 2% glutamine attenuates these changes. This experiment examines whether a 2% glycyl‐L‐glutamine‐enriched TPN solution reverses IV TPN‐induced changes as effectively as L‐glutamine. Methods: Male Institute of Cancer Research (ICR) mice underwent intranasal inoculation with H1N1 influenza virus to establish immunity. After 3 weeks, mice were randomized to chow, IV feeding of a TPN solution, glutamine‐enriched TPN, or glycyl‐L‐glutamine‐enriched TPN. After 4 days of feeding, mice were challenged intranasally with influenza virus and killed at 40 hours to determine viral shedding from the respiratory tract; normal convalescent mice do not shed virus because they possess intact IgA‐mediated mechanisms. Lymphocytes were isolated from Peyer's patches, the intraepithelial layer, and lamina propria to determine cell yields. Results: Total lymphocyte yield in the Peyer's patches, the intraepithelial layer, and lamina propria decreased with TPN but remained normal with glutamine and glycyl‐L‐glutamine. Upon challenge, 70% of the mice in the TPN group shed virus in nasal secretions, whereas only 20% of the glutamine‐treated group, 18% of glycyl‐L‐glutamine group and none of the Chow group were virus positive. Conclusions: L‐Glutamine and glycyl‐L‐glutamine have similar effects on IV administered TPN‐associated (GALT) atrophy and decreased upper respiratory tract immunity. ( Journal of Parenteral and Enteral Nutrition 22 :31–36, 1998)
Type of Medium:
Online Resource
ISSN:
0148-6071
,
1941-2444
DOI:
10.1177/014860719802200131
Language:
English
Publisher:
Wiley
Publication Date:
1998
detail.hit.zdb_id:
800861-9
detail.hit.zdb_id:
2170060-6
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