In:
Journal of Bone and Mineral Research, Wiley, Vol. 14, No. 10 ( 1999-10), p. 1761-1767
Abstract:
We previously showed that sphingosine 1‐phosphate acts as a second messenger for tumor necrosis factor α–induced interleukin‐6 synthesis in osteoblast‐like MC3T3‐E1 cells and that the synthesis by sphingosine 1‐phosphate is dependent on p42/p44 mitogen‐activated protein (MAP) kinase activation. In the present study, we investigated the effect of sphingosine 1‐phosphate on the induction of heat shock protein 27 (HSP27) in MC3T3‐E1 cells. Not C 2 ‐ceramide, but sphingosine and sphingosine 1‐phosphate significantly induced HSP27 accumulation dose dependently in the range between 1μM and 30 μM. DL‐threo‐dihydrosphingosine, an inhibitor of sphingosine kinase, markedly inhibited the sphingosine‐induced HSP27 accumulation. Sphingosine 1‐phosphate induced increase in the levels of the mRNA for HSP27. Sphingosine 1‐phosphate stimulated the phosphorylation of p38 MAP kinase. The sphingosine 1‐phosphate–induced HSP27 accumulation was dose dependently suppressed by SB203580, an inhibitor of p38 MAP kinase, but not PD98059, an inhibitor of the upstream kinase that activates p42/p44 MAP kinase. SB203580 reduced the sphingosine 1‐phosphate–induced increase of mRNA for HSP27. These results strongly suggest that sphingosine 1‐phosphate–stimulated HSP27 induction is mediated via p38 MAP kinase activation in osteoblasts.
Type of Medium:
Online Resource
ISSN:
0884-0431
,
1523-4681
DOI:
10.1359/jbmr.1999.14.10.1761
Language:
English
Publisher:
Wiley
Publication Date:
1999
detail.hit.zdb_id:
2008867-X
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