In:
Hypertension, Ovid Technologies (Wolters Kluwer Health), Vol. 27, No. 6 ( 1996-06), p. 1312-1317
Abstract:
Abstract We have reported that cytosolic Ca 2+ concentration ([Ca 2+ ] i ) is increased in platelets from spontaneously hypertensive rats (SHR) in both basal and thrombin-stimulated conditions. To determine whether the correlation between blood pressure and cellular Ca 2+ metabolism exists in stroke-prone SHR (SHRSP), we investigated Ca 2+ handling using fura 2 and aggregation response in platelets of 12- to 13-week-old male SHRSP, SHR, and Wistar-Kyoto rats (WKY). Systolic pressure was highest in SHRSP and lowest in WKY (213±8, 172±7, and 135±5 mm Hg, respectively). Basal [Ca 2+ ] i was significantly higher in SHR than WKY (45.9±4.5 versus 41.2±4.8 nmol/L, P 〈 .05), and that in SHRSP (40.2±2.8 nmol/L) was similar to that in WKY. Thrombin (0.1 IU/mL)–stimulated [Ca 2+ ] i rise was greater in SHR and smaller in SHRSP than in WKY in the presence of extracellular Ca 2+ (530±50 and 408±52 versus 475±50 nmol/L, respectively; P 〈 .05). The recovery rate from the peak [Ca 2+ ] i response to thrombin was greatest in SHRSP and least in WKY. Ionomycin (5 μmol/L)–stimulated [Ca 2+ ] i rise was similar in WKY, SHR, and SHRSP (731±97, 743±88, and 683±70 nmol/L, respectively). Thrombin-induced maximum platelet aggregation response was higher in SHR and lower in SHRSP than WKY (82±4% and 61±15% versus 73±6%, respectively; P 〈 .05). In contrast to SHR, basal [Ca 2+ ] i in SHRSP was similar to that in WKY, and thrombin-stimulated [Ca 2+ ] i was attenuated. These results suggest that platelet Ca 2+ handling differs between SHR substrains and that an increased [Ca 2+ ] i is not obligatory in genetically hypertensive rats.
Type of Medium:
Online Resource
ISSN:
0194-911X
,
1524-4563
DOI:
10.1161/01.HYP.27.6.1312
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
1996
detail.hit.zdb_id:
2094210-2
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