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  • 1
    Online Resource
    Online Resource
    Cloning and characterization of LRG5 , a gene involved in blue light signaling in Chlamydomonas gametogenesis
    Wiley ; 1997
    In:  The Plant Journal Vol. 12, No. 3 ( 1997-09), p. 677-683
    Details
    In: The Plant Journal, Wiley, Vol. 12, No. 3 ( 1997-09), p. 677-683
    Abstract: The Chlamydomonas reinhardtii mutant lsrg2 exhibits a partial defect in the blue‐light activated signal transduction chain that controls gametogenesis. By genomic complementation, a cosmid clone that corrects the Irg2 phenotype was isolated. The smallest fragment of this cosmid that gave complementation was used in RFLP analysis, which revealed that the cloned gene was not linked to the LRG2 locus. The sequence of the genomic clone and a cDNA of this gene, which we named LRG5 , was determined. Although no significant homology with sequences in the databases was found, the nuclear localization signal and the presence of negatively and positively charged domains suggests a function for the LRG5 protein in the regulation of transcription. The correction of the Irg2 phenotype is probably caused by overexpression of the LRG5 gene in the transformants, as these expressed a level of LRG5 approximately twofold higher than wild‐type or mutant strains. Southern blot analysis provided evidence for the existence of homologous sequences in other green algae as well as in higher plants, suggesting a conserved function for the LRG5 protein.
    Type of Medium: Online Resource
    ISSN: 0960-7412 , 1365-313X
    URL: Article
    DOI: 10.1046/j.1365-313X.1997.00677.x
    Language: English
    Publisher: Wiley
    Publication Date: 1997
    detail.hit.zdb_id: 2020961-7
    SSG: 12
    Location Call Number Limitation Availability
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Cloning and characterization of LRG5 , a gene involved in blue light signaling in Chlamydomonas gametogenesis
    Wiley ; 1997
    In:  The Plant Journal Vol. 12, No. 3 ( 1997-09), p. 677-683
    Details
    In: The Plant Journal, Wiley, Vol. 12, No. 3 ( 1997-09), p. 677-683
    Abstract: The Chlamydomonas reinhardtii mutant lrg2 exhibits a partial defect in the blue‐light activated signal transduction chain that controls gametogenesis. By genomic complementation, a cosmid clone that corrects the lrg2 phenotype was isolated. The smallest fragment of this cosmid that gave complementation was used in RFLP analysis, which revealed that the cloned gene was not linked to the LRG2 locus. The sequence of the genomic clone and a cDNA of this gene, which we named LRG5 , was determined. Although no significant homology with sequences in the databases was found, the nuclear localization signal and the presence of negatively and positively charged domains suggests a function for the LRG5 protein in the regulation of transcription. The correction of the lrg2 phenotype is probably caused by overexpression of the LRG5 gene in the transformants, as these expressed a level of LRG5 approximately twofold higher than wild‐type or mutant strains. Southern blot analysis provided evidence for the existence of homologous sequences in other green algae as well as in higher plants, suggesting a conserved function for the LRG5 protein.
    Type of Medium: Online Resource
    ISSN: 0960-7412 , 1365-313X
    URL: Article
    DOI: 10.1046/j.1365-313X.1997.d01-20.x
    Language: English
    Publisher: Wiley
    Publication Date: 1997
    detail.hit.zdb_id: 2020961-7
    SSG: 12
    Location Call Number Limitation Availability
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  • 3
    Online Resource
    Online Resource
    Large-Scale Sequencing of Two Regions in Human Chromosome 7q22: Analysis of 650 kb of Genomic Sequence around the EPO and CUTL1 Loci Reveals 17 Genes
    Cold Spring Harbor Laboratory ; 1998
    In:  Genome Research Vol. 8, No. 10 ( 1998-10-01), p. 1060-1073
    Details
    In: Genome Research, Cold Spring Harbor Laboratory, Vol. 8, No. 10 ( 1998-10-01), p. 1060-1073
    Abstract: We have sequenced and annotated two genomic regions located in the Giemsa negative band q22 of human chromosome 7. The first region defined by the erythropoietin ( EPO ) locus is 228 kb in length and contains 13 genes. Whereas 3 genes ( GNB2, EPO , PCOLCE ) were known previously on the mRNA level, we have been able to identify 10 novel genes using a newly developed automatic annotation tool RUMMAGE-DP, which comprises 〉 26 different programs mainly for exon prediction, homology searches, and compositional and repeat analysis. For precise annotation we have also resequenced ESTs identified to the region and assembled them to build large cDNAs. In addition, we have investigated the differential splicing of genes. Using these tools we annotated 4 of the 10 genes as a zonadhesin, a transferrin homolog, a nucleoporin-like gene, and an actin gene. Two genes showed weak similarity to an insulin-like receptor and a neuronal protein with a leucine-rich amino-terminal domain. Four predicted genes ( CDS1–CDS4 ) CDS that have been confirmed on the mRNA level showed no similarity to known proteins and a potential function could not be assigned. The second region in 7q22 defined by the CUTL1 (CCAAT displacement protein and its splice variant) locus is 416 kb in length and contains three known genes, including PMSL12, APS, CUTL1 , and a novel gene ( CDS5 ). The CUTL1 locus, consisting of two splice variants ( CDP and CASP ), occupies 〉 300 kb. Based on the G,C profile an isochore switch can be defined between the CUTL1 gene and the APS and PMSL12 genes. [Clones 37G3, 164c7, and 235f8 are deposited in GenBank under accession no. AF053356 ; clone 123e15, accession no. AF024533 ; 186d2, accession no. AF024534 ; 46f6, accession no. AF006752 ; 50h2, accession no. AF047825 ; and 76h2, accession no. AF030453 ]
    Type of Medium: Online Resource
    ISSN: 1088-9051 , 1549-5469
    URL: Article
    DOI: 10.1101/gr.8.10.1060
    RVK:
    XA 10000
    Language: English
    Publisher: Cold Spring Harbor Laboratory
    Publication Date: 1998
    detail.hit.zdb_id: 1483456-X
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Genomic Structure of a Novel LIM Domain Gene (ZNF185) in Xq28 and Comparisons with the Orthologous Murine Transcript
    Elsevier BV ; 1997
    In:  Genomics Vol. 43, No. 3 ( 1997-08), p. 329-338
    Details
    In: Genomics, Elsevier BV, Vol. 43, No. 3 ( 1997-08), p. 329-338
    Type of Medium: Online Resource
    ISSN: 0888-7543
    URL: Article
    DOI: 10.1006/geno.1997.4810
    RVK:
    XA 10000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 1997
    detail.hit.zdb_id: 1468023-3
    SSG: 12
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