In:
Genes & Development, Cold Spring Harbor Laboratory, Vol. 12, No. 15 ( 1998-08-01), p. 2305-2317
Abstract:
Deletion of the TCRβ transcriptional enhancer (Eβ) results in nearly complete inhibition of V(D)J recombination at the TCRβ locus and a block in αβ T cell development. This result, along with previous work from many laboratories, has led to the hypothesis that transcriptional enhancers affect V(D)J recombination by regulating the accessibility of the locus to the recombinase. Here we test this hypothesis by performing a detailed analysis of the recombination defect in Eβ-deleted (Eβ −/− ) mice using assays that detect various reaction intermediates and products. We found double-strand DNA breaks at recombination signal sequences flanking D β and J β gene segments in Eβ −/− thymuses at about one-third to one-thirtieth the level found in thymuses with an unaltered TCRβ locus. These sites are also subject to in vitro cleavage by the V(D)J recombinase in both Eβ −/− and Eβ +/+ thymocyte nuclei. However, the corresponding D β-to- J β coding joints are further reduced (by 100- to 300-fold) in Eβ −/− thymuses. Formation of extrachromosomal D β-to- J β signal joints appears to be intermediately affected and nonstandard D β-to- D β joining occurs at the Eβ-deleted alleles. These data indicate that, unexpectedly, loss of accessibility alone cannot explain the loss of TCRβ recombination in the absence of the Eβ element and suggest an additional function for Eβ in the process of DNA repair at specific TCRβ sites during the late phase of the recombination reaction.
Type of Medium:
Online Resource
ISSN:
0890-9369
,
1549-5477
DOI:
10.1101/gad.12.15.2305
Language:
English
Publisher:
Cold Spring Harbor Laboratory
Publication Date:
1998
detail.hit.zdb_id:
1467414-2
SSG:
12
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