In:
Reproduction, Fertility and Development, CSIRO Publishing, Vol. 11, No. 8 ( 1999), p. 387-
Abstract:
The meiotic competence of canine oocytes cultured for 72 h in medium
supplemented with three different concentrations (5, 10 and 20%) of anoestrous, oestrous or metoestrous bitch serum, or with 0.3% bovine
serum albumin (BSA), was examined. The oestrous serum supplement had a positive effect on the resumption of meiosis, compared with the other
supplements (P 〈 0.05). The number of oocytes that
reached metaphase I (MI) and metaphase II (MII) was significantly higher (P 〈 0.05) with the oestrous serum supplement than with
the anoestrous serum supplement. There were no significant differences among the three different concentrations in each serum type with respect to the
proportion of oocytes that completed meiosis (MI to MII). The number of oocytes that resumed meiosis in the 10% oestrous serum supplement was
significantly higher (P 〈 0.05) than those of each concentration of the anoestrous and metoestrous serum supplements, and of the
0.3% BSA supplement. Moreover, a higher number of oocytes reached MII in the presence of the 10% oestrous serum supplement than with the
10% anoestrous serum supplement. These results suggest that supplementation of the culture medium with 10% oestrous serum is the
optimal treatment for in vitro maturation of canine oocytes.
Type of Medium:
Online Resource
ISSN:
1031-3613
Language:
English
Publisher:
CSIRO Publishing
Publication Date:
1999
SSG:
12
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