In:
Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures Processing, Measurement, and Phenomena, American Vacuum Society, Vol. 15, No. 5 ( 1997-09-01), p. 1637-1640
Abstract:
λ-DNA Hind III was denatured by HCl, renatured by NaOH, and completely digested by DNase I sequentially. The produced mixture was separated by gel filtration, and one component was obtained which showed resistance to the digestion of DNase 1. The results of atomic force microscopy (AFM) studies on this component indicated that an unusual form of DNA was presented, with the height 13 times that of double-stranded DNA. The renatured and partially digested λ-DNA Hind III was also studied by AFM, and the images revealed both the duplex DNA and the unusual DNA. By comparison with known results, it is suggested that the unusual DNA is a multistranded DNA rather than a simple supercoil.
Type of Medium:
Online Resource
ISSN:
1071-1023
,
1520-8567
Language:
English
Publisher:
American Vacuum Society
Publication Date:
1997
detail.hit.zdb_id:
3117331-7
detail.hit.zdb_id:
3117333-0
detail.hit.zdb_id:
1475429-0
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