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  • Industrial Chemistry  (13)
  • phospholamban
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1052-1054 
    ISSN: 1420-9071
    Keywords: Cardiac muscle ; phylogenesis ; sarcoplasmic reticulum ; phospholamban ; protein kinase ; Ca2+-transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Phospholamban, a sarcoplasmic reticulum phosphoprotein, is present in the hearts of mammalian, avian, amphibian, and fish species. Phylogenetic changes are indicated by marked differences among species in cardiac phospholamban content and by the absence of Ca2+/calmodulin-dependent phospholamban phosphorylation at an early developmental stage.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-4919
    Keywords: neonatal rat cardiomyocyte culture ; sarcoplasmic reticulum ; phospholamban ; calcium ATPase ; calcium transport ; thyroid hormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract This study investigates sarcoplasmic reticulum (SR) calcium-(Ca2+) transport ATPase (SERCA2a) and phospholamban (PLB) in cultured spontaneously contracting neonatal rat cardiomyocytes (CM) to ascertain the function of both SR proteins under various culture conditions. The two major SR proteins were readily detectable in cultured CM by immunofluorescent microscopy using specific anti-SERCA2 and anti-PLB antibodies. Double labeling technique revealed that PLB-positive CM also labeled with anti-SERCA2. Coexpression of SERCA2 and PLB in CM was supported by measurement of cell homogenate oxalate-supported Ca2+ uptake which was completely inhibited by thapsigargin and stimulated by protein kinase A-catalyzed phosphorylation. Under serum-free conditions, incubation of CM with the SERCA2a expression modulator 3,3′,5-triiodo-L-thyronine (100 nM, 72 h) resulted in elevated Ca2+ uptake of +33%. Specific Ca2+ uptake activity was not altered if insulin was omitted from the serum-free culture medium but total SR Ca2+ transport activity was reduced under this culture condition. The results indicate that primary culture of spontaneously contracting neonatal rat CM can be employed as a useful model system for investigating both short- and long-term mechanisms determining the Ca2+ re-uptake function of the SR under defined culture conditions.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-4919
    Keywords: heart ; postnatal development ; sarcoplasmic reticulum ; phospholamban ; calcium transport ; spontaneously hypertensive rats ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract This comparative study investigates the relationship between sarcoplasmic reticulum (SR) calcium(Ca2+)-ATPase transport activity and phospholamban (PLB) phosphorylation in whole cardiac homogenates of spo`ntaneously hypertensive rats (SHR) and their parent, normotensive Wistar Kyoto (WKY) strain during early postnatal development at days 1, 3, 6, 12 and at day 40 to ascertain any difference in SR Ca2+ handling before the onset of hypertension. At day 1, the rate of homogenate oxalate-supported Ca2+ uptake was significantly higher in SHR than in WKY (0.25 ± 0.02 vs 0.12 ± 0.01 nmoles Ca2+/mg wet ventricular weight/min, respectively; p 〈 0.001). This interstrain difference disappeared with further developmental increase in SR Ca2+ transport. Western Blot analysis and a semiquantitative ELISA did not reveal any difference in the amount of immunoreactive PLB (per mg of total tissue protein) between strains at any of the ages studied. In addition, levels of phosphorylated PLB formed in vitro in the presence of radiolabelled ATP and catalytic (C) subunit of protein kinase A did not differ between SHR and WKY at days 1, 3, 6 and 12. At day 40, C subunit-catalyzed formation of 32P-PLB was reduced by 66% (p 〈 0.001) in SHR when compared to age-matched WKY In the early postnatal period between day 1 and 12 SR Ca2+-transport values were linearly related to the respective 32P-PLB levels of both SHR and WKY rats. The results indicate that cardiac SR of SHR can sequester Ca2+ at a much higher rate immediately after birth compared to WKY rats. The disappearance of this interstrain difference with further development suggests that some endogenous neuroendocrine or nutritional factor(s) from the hypertensive mother may exert an influence upon the developing heart in utero resulting in a transiently advanced maturation of the SR Ca2+ transport function in SHR pups at the time of birth.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-4919
    Keywords: atria ; thyroid hormones ; β-adrenergic effect ; sarcoplasmic reticulum ; phospholamban
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract This paper discusses the mechanisms of two basic effects of thyroid hormones on atrial responses to β-adrenergic agonists, i.e. increased inotropic sensitivity and decreased maximal contractile responsiveness. The increased sensitivity of atria to β-adrenergic agonists under thyroid hormones appears to be related to increases in β-adrenoceptor density and Gs/Gi protein ratio, leading to activation of Gs-mediated pathway, but suppression of Gi-mediated pathway of adenylate cyclase regulation. Therefore, the i/c concentrations of cAMP and corresponding inotropic responses achieve their maximums at lower doses of β-adrenergic agonist. Thyroid hormones also decrease the expression of phospholamban, but increase the expression of sarcoplasmic reticulum Ca+2-pump. As a result, the basal activity of sarcoplasmic reticulum Ca+2-pump increases, but its β-adrenergic activation through phosphorylation of phospholamban decreases. It is suggested that these changes are causal for decreased maximal inotropic and lusitropic responses of atria to β-adrenergic agonists.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemical Engineering & Technology - CET 10 (1987), S. 262-271 
    ISSN: 0930-7516
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pressure pulsations in hydraulic systems, generated by reciprocating pumps, can cause serious problems with regard to plant safety and reliability. In particular, fatigue problems arise in high-pressure piping systems. The available knowledge is not sufficient for an accurate computation of pressure peaks in the piping of reciprocating pumps. A number of calculation models are available which, however, neglect both fluid compressibility and friction. This contribution presents a calculation method which allows a precise modelling of various pump installations. Comparison of calculated and experimental data shows a good agreement and provides a validation of the computational model.
    Additional Material: 18 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 36 (1964), S. 185-191 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Die Anwendungsmöglichkeiten von Dosierpumpen in der Verfahrenstechnik werden durch Fördermedium, Druck, Temperatur und Fördermenge bestimmt. Das Förderverhalten oszillierender Verdrängerpumpen ist vor allem von der Kompressibilität, der Viscosität und dem Dampfdruck des Fördermediums abhängig. Untersuchungen zur Ermittlung der Dosiergenauigkeit bei verschiedenen Bedingungen zeigen, daß Dosierpumpen bei geeigneter Ausführung und passenden Randbedingungen genau arbeiten. Für die Gewährleistung der Dosiergenauigkeit sind Maßnahmen erforderlich, um den Verschleiß niedrig zu halten. Besondere Anforderungen stellen Entwicklung und Bau von Dosierpumpen für Drucke bis 3000 at.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Schwellende Innendruckbeanspruchungen treten in Behältern, Reaktoren, Rohrleitungen, Armaturen, Pumpen und Verdichtern auf. Bei Apparaten wird die schwellende Beanspruchung oft nur durch die Inbetriebnahme und den Abschaltvorgang verursacht, so daß die Lastspielzahl innerhalb der Lebenszeit des Bauteils klein ist. In diesen Fällen werden die Bauteile nach der Zeitfestigkeit ausgelegt. Bei Pumpen und Verdichtern jedoch ist die Lastspielzahl sehr hoch, so daß, abgesehen von wenigen Ausnahmen, eine Auslegung auf Dauerfestigkeit erforderlich ist. Die Bauteile des Pumpen- und Verdichterbaues, welche im Dauerbetrieb durch schwellenden Innendruck belastet sind, sowie die dafür verwendeten Werkstoffe werden auf den Hochdruckmaschinenbau abgestimmt, weshalb nur verhältnismäßig dickwandige Bauteile behandelt werden. Es werden Versuchsergebnisse mitgeteilt und Hinweise für die Auslegung, die Wahl des Werkstoffes und die Oberflächenbehandlung gegeben.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An einer Kolbenpumpe mit Acrylglas-Zylinder wird das Verhalten der Ventile ohne Kavitation und bei verschiedener Stärke der Dampfblasenbildung beobachtet und anhand von Ventilhub- und Druckabfallkurven diskutiert. Die Ergebnisse werden mit stationären Messungen verglichen. Es zeigt sich, daß bei nichtkavitierendem Pumpenbetrieb der Druckabfall im Saugventil aus den bei stationärer Strömung gemessenen Verlustbeiwerten ζv = f (Re) berechnet werden kann. Abschließend werden die Auswirkungen verschiedener Kavitationszustände auf das Betriebsverhalten der Pumpe beschrieben und Maßnahmen zur Vermeidung schädlicher Kavitation genannt.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 42 (1970), S. 609-616 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Zur Senkung von Druck- und Förderstromschwankungen pulsierender Flüssigkeitsströme, wie sie bei oszillierenden Verdrängerpumpen auftreten, werden gasgefüllte Pulsationsdämpfer (Windkessel) verwendet. Ihre Wirkungsweise wird am Modell eines fremderregten Schwingungssystems erläutert, wobei die exakte Lösung zu einer nichtlinearen, inhomogenen Differentialgleichung 2. Ordnung führt. Für die in der Praxis interessierenden Betriebsbereiche eines Pulsationsdämpfers werden Näherungslösungen angegeben, welche es gestatten, die Auslegungsberechnungen ohne großen Aufwand vorzunehmen.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 35 (1963), S. 267-272 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Obwohl Dosierpumpen in der gesamten Verfahrenstechnik häufig verwendet werden, sind systematische Untersuchungen über die Genauigkeit der Dosieraggregate nicht bekannt geworden. Die vorliegende Arbeit berichtet über meßtechnische Probleme und Versuche bei der Ermittlung der DosiergenauigkeitDie Messungen wurden im Laboratorium der Firma Lewa, Herbert Ott KG., Leonberg, ausgeführt.. Zunächst werden die Begriffe Dosiergenauigkeit, Reproduzierbarkeit und Einstellgenauigkeit und die Methode der Fehlerangabe erläutert. Die mit einer Pumpe bei veränderlicher Hublänge und verschiedenen Drucken im Bereich zwischen 2,5 und 127 atü bei reinem Fördermedium gewonnenen Ergebnisse zeigen, daß unter günstigen Bedingungen Dosierpumpen über einen Mengenbereich von mindestens 1 : 50 mit einem Fehler von weniger als ± 0,1%, bezogen auf die maximale Fördermenge, arbeiten können. Damit erweist sich die Dosierpumpe als ein auch bei großem Regelbereich außerordentlich genaues Gerät.
    Additional Material: 21 Ill.
    Type of Medium: Electronic Resource
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