ISSN:
1615-6102
Keywords:
Arabidopsis thaliana
;
Glyceraldehyde-3-phosphate dehydrogenase
;
Antibody
;
Phloem
;
Sieve element
;
Companion cell
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (NAD-dependent GAPDH) was purified to homogeneity and injected into a rabbit to induce a polyclonal antibody. The antibody was judged to be of high specificity and high affinity. This antibody was used to probe sections ofArabidopsis leaf, stem or roots which were fixed using either paraformaldehyde or a high-pressure freezing method. Our results show that the NAD-dependent GAPDH localizes in the nucleus as well as in the cytosol. In phloem tissue, the NAD-dependent GAPDH was found in companion cells but not in the sieve element.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01287564
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